Housing conditions differentially affect physiological and behavioural stress responses of zebrafish, as well as the response to anxiolytics.
Housing conditions differentially affect physiological and behavioural stress responses of zebrafish, as well as the response to anxiolytics.
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DOI：
10.1371/journal.pone.0034992
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Brennan CH
中科院分区：
文献类型：
--
作者：
Parker MO;Millington ME;Combe FJ;Brennan CH
斑马鱼是发育遗传学中广泛使用的动物模型，由于我们对斑马鱼行为的理解最近取得了进展，它们作为行为神经科学中的比较模型的作用开始被认识到。一种被广泛报道的行为测试是新的坦克潜水测试，它经常被引用为焦虑和压力反应的测试。尽管它被广泛使用，并针对抗焦虑药物进行了各种验证，但关于测试前住房的报道在文献中一直很少。由于斑马鱼是浅水型物种，我们预测栖息地环境会影响它们的应激反应，从而影响它们在坦克潜水过程中的反应。在我们的第一个实验中，我们测试了住房的各个方面(大群体，大群体没有接触，配对，只有视觉接触，只有嗅觉接触)，发现坦克潜水反应是通过与同种生物的视觉接触来调节的。我们还测试了群体和个体饲养的鱼的基础皮质醇水平，发现个体饲养的个体的基础皮质醇水平较低。在我们的第二个实验中，我们发现乙醇似乎对单独饲养的鱼有缓解焦虑的作用，但对集体饲养的鱼没有。在我们的最后一个实验中，我们检查了在鱼缸潜水之前改变鱼的水作为额外的驯化程序的效果。我们发现，这对单独安置的鱼没有影响，但似乎影响了集体安置的个体的典型鱼缸潜水反应。总之，我们证明住房是从这种方法中获得可靠数据的一个重要因素，对斑马鱼焦虑的比较模型感兴趣的研究人员应该考虑住房，以便改进他们的方法，并增加他们在实验中的能力。
Zebrafish are a widely utilised animal model in developmental genetics, and owing to recent advances in our understanding of zebrafish behaviour, their utility as a comparative model in behavioural neuroscience is beginning to be realised. One widely reported behavioural measure is the novel tank-diving assay, which has been often cited as a test of anxiety and stress reactivity. Despite its wide utilisation, and various validations against anxiolytic drugs, reporting of pre-test housing has been sparse in the literature. As zebrafish are a shoaling species, we predicted that housing environment would affect their stress reactivity and, as such, their response in the tank-diving procedure. In our first experiment, we tested various aspects of housing (large groups, large groups with no contact, paired, visual contact only, olfactory contact only) and found that the tank diving response was mediated by visual contact with conspecifics. We also tested the basal cortisol levels of group and individually housed fish, and found that individually housed individuals have lower basal cortisol levels. In our second experiment we found ethanol appeared to have an anxiolytic effect with individually housed fish but not those that were group housed. In our final experiment, we examined the effects of changing the fishes' water prior to tank diving as an additional acclimation procedure. We found that this had no effect on individually housed fish, but appeared to affect the typical tank diving responses of the group housed individuals. In conclusion, we demonstrate that housing represents an important factor in obtaining reliable data from this methodology, and should be considered by researchers interested in comparative models of anxiety in zebrafish in order to refine their approach and to increase the power in their experiments.
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影响因子：
2.7
作者：
Egan, Rupert J.;Bergner, Carisa L.;Hart, Peter C.;Cachat, Jonathan M.;Canavello, Peter R.;Elegante, Marco F.;Elkhayat, Salem I.;Bartels, Brett K.;Tien, Anna K.;Tien, David H.;Mohnot, Sopan;Beeson, Esther;Glasgow, Eric;Amri, Hakima;Zukowska, Zofia;Kalueff, Allan V.
通讯作者：
Kalueff, Allan V.
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3.7
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Dahlbom, S. Josefin;Lagman, David;Winberg, Svante
通讯作者：
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2.4
作者：
Norton W;Bally-Cuif L
通讯作者：
Bally-Cuif L
影响因子：
2.7
作者：
Parker, Matthew O.;Millington, Mollie E.;Brennan, Caroline H.
通讯作者：
Brennan, Caroline H.
影响因子：
4.5
作者：
Ramsay, Jennifer M.;Feist, Grant W.;Schreck, Carl B.
通讯作者：
Schreck, Carl B.