Bacterial artificial chromosome clones of viruses comprising the towne cytomegalovirus vaccine.
Bacterial artificial chromosome clones of viruses comprising the towne cytomegalovirus vaccine.
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DOI：
10.1155/2012/428498
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发表时间：
2012
影响因子：
--
通讯作者：
McVoy MA
中科院分区：
文献类型：
--
作者：
Cui X;Adler SP;Davison AJ;Smith L;Habib el-SE;McVoy MA
细菌人工染色体（BAC）克隆已被证明是非常宝贵的遗传操作疱疹病毒基因组。BAC克隆也可用于捕获包含病毒原液或混合物的代表性基因组。Towne减毒活巨细胞病毒疫苗是在20世纪70年代通过在培养的成纤维细胞中连续传代而开发的。虽然它的安全性，免疫原性和有效性已经在近1000名人类受试者中进行了评估，但疫苗本身的研究很少。相反，遗传组成和体外生长特性是从实验室储备的研究中推断出来的，这些研究可能并不总是准确地代表构成疫苗的病毒。在这里，我们描述了使用BAC克隆来定义来自Towne疫苗的病毒的基因型和表型特性。鉴于Towne疫苗广泛的安全历史，这些BAC为下一代合理工程化巨细胞病毒疫苗的开发提供了逻辑起点。
Bacterial artificial chromosome (BAC) clones have proven invaluable for genetic manipulation of herpesvirus genomes. BAC cloning can also be useful for capturing representative genomes that comprise a viral stock or mixture. The Towne live attenuated cytomegalovirus vaccine was developed in the 1970s by serial passage in cultured fibroblasts. Although its safety, immunogenicity, and efficacy have been evaluated in nearly a thousand human subjects, the vaccine itself has been little studied. Instead, genetic composition and in vitro growth properties have been inferred from studies of laboratory stocks that may not always accurately represent the viruses that comprise the vaccine. Here we describe the use of BAC cloning to define the genotypic and phenotypic properties of viruses from the Towne vaccine. Given the extensive safety history of the Towne vaccine, these BACs provide a logical starting point for the development of next-generation rationally engineered cytomegalovirus vaccines.
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影响因子：
5.5
作者：
Saccoccio, Frances M.;Sauer, Anne L.;Cui, Xiaohong;Armstrong, Amy E.;Habib, El-Sayed E.;Johnson, David C.;Ryckman, Brent J.;Klingelhutz, Aloysius J.;Adler, Stuart P.;McVoy, Michael A.
通讯作者：
McVoy, Michael A.
影响因子：
3.8
作者：
Davison, AJ;Dolan, A;Hayward, GS
通讯作者：
Hayward, GS
影响因子：
3.7
作者：
Cui, Xiaohong;McGregor, Alistair;Schleiss, Mark R.;McVoy, Michael A.
通讯作者：
McVoy, Michael A.
影响因子：
5.4
作者：
Macagno, Annalisa;Bernasconi, Nadia L.;Lanzavecchia, Antonio
通讯作者：
Lanzavecchia, Antonio
影响因子：
3.8
作者：
Dolan, A;Cunningham, C;Davison, AJ
通讯作者：
Davison, AJ