Effects of endophytic fungi on parasitic process of Taxillus chinensis.
Effects of endophytic fungi on parasitic process of Taxillus chinensis.
复制标题
内生真菌对红豆杉寄生过程的影响。
DOI：
10.1038/s41598-022-11940-z
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发表时间：
2022-05-11
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
中国杉（Taxillus chinensis）丹参是一种在传统和现代药物系统中广泛使用的药用灌木。它是一种多年生半寄生植物，由于其寄生率低，很难人工繁殖。寄生植物成功的寄生是将自身的组织融合，并将自身的脉管系统与寄主的脉管系统连接起来，建立起一座生理桥梁，有效地从寄主体内吸收水分、糖分和营养物质。据报道，内生真菌在细胞壁降解和融合中起着重要作用，这是生理桥梁形成的关键过程。因此，本研究首先分离了不同寄主的内生真菌，然后用愈创木酚和纤维素降解能力组成的培养基筛选出能够降解主要细胞壁成分的微生物。结果表明，从72株柽柳内生真菌中筛选出5株具有高降解木质纤维素酶活性的内生真菌。5株菌株的漆酶和纤维素酶活性在第7天达到峰值，其中菌株P6的漆酶和纤维素酶活性最高，分别为117.66和1.66 U/mL。菌株4的锰过氧化物酶和菌株N6的木质素过氧化物酶也在第7天达到峰值，在5株菌株中最高，酶活性分别为11.61和6.64 U/mL。菌株4、15、31、N6和P6根据形态和分子生物学特性分别鉴定为炭疽菌（Colletotrichum sp.）、球状黑孢菌（Nigerrospora sphaerica）、芽孢菌（exserhilum sp.）、phaseolorum Diaporthe和拟盘多毛孢（pstalotiopsis sp.）。内生真菌可分泌有效的细胞壁降解酶，促进寄主与赤霉素之间细胞壁的溶解和松弛，从而促进赤霉素的寄生。
Taxillus chinensis (DC.) Danser is an extensively used medicinal shrub in the traditional as well as modern systems of medicines. It is a perennial hemiparasitic plant, which is difficult to propagate artificially because of its low parasitic rate. Successful parasitism of parasitic plants is to fuse their tissues and connect their vasculature to the host vasculature building a physiological bridge, which can efficiently withdraw water, sugars and nutrients from their host plants. It is reported that endophytic fungi play an important role in cell wall degradation and fusion, which is the key forming process of the physiological bridge. Therefore, in this study, the endophytic fungi from T. chinensis of different hosts were isolated, and then the organisms that could degrade the main components of the cell walls were screened out using a medium consisting of guaihuol and cellulose degradation capacity. The results showed that five strains were screened out from 72 endophytic fungi of T. chinensis which with high enzyme activities for lignocellulosic degradation. The laccase and cellulase activities of five strains reached their peaks at day 7, and the highest enzyme activities of these two enzymes were found in strain P6, which was 117.66 and 1.66 U/mL, respectively. Manganese peroxidase of strain 4 and lignin peroxidase of strain N6 also reached their peaks at day 7 and were the highest among the 5 strains, with enzyme activities of 11.61 and 6.64 U/mL, respectively. Strains 4, 15, 31, N6 and P6 were identified as Colletotrichum sp., Nigerrospora sphaerica, Exserohilum sp., Diaporthe phaseolorum and Pestalotiopsis sp., respectively, according to their morphological and molecular biology properties. The endophytic fungi may secrete efficient cell wall degradation enzymes, which promote the dissolution and relaxation of the cell wall between T. chinensis and host, thus contributing to the parasitism of T. chinensis.
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编码植物细胞壁降解酶的基因从植物相关宿主大规模横向转移到真菌寄生真菌木霉属
影响因子：
4.5
作者：
Druzhinina IS;Chenthamara K;Zhang J;Atanasova L;Yang D;Miao Y;Rahimi MJ;Grujic M;Cai F;Pourmehdi S;Salim KA;Pretzer C;Kopchinskiy AG;Henrissat B;Kuo A;Hundley H;Wang M;Aerts A;Salamov A;Lipzen A;LaButti K;Barry K;Grigoriev IV;Shen Q;Kubicek CP
通讯作者：
Kubicek CP
影响因子：
9.4
作者：
LeBlanc, Megan;Kim, Gunjune;Westwood, James
通讯作者：
Westwood, James
影响因子：
1.6
作者：
Harnpicharnchai, Piyanun;Champreda, Verawat;Eurwilaichitr, Lily
通讯作者：
Eurwilaichitr, Lily
影响因子：
3.4
作者：
Hakala, TK;Lundell, T;Hatakka, A
通讯作者：
Hatakka, A
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro