Characterization of the Heavy-Metal-Associated Isoprenylated Plant Protein (HIPP) Gene Family from Triticeae Species.
Characterization of the Heavy-Metal-Associated Isoprenylated Plant Protein (HIPP) Gene Family from Triticeae Species.
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DOI：
10.3390/ijms21176191
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发表时间：
2020-08-27
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Zhang H;Zhang X;Liu J;Niu Y;Chen Y;Hao Y;Zhao J;Sun L;Wang H;Xiao J;Wang X
重金属相关异戊二烯化植物蛋白（HMA）仅存在于维管植物中。它们在响应生物/非生物胁迫、重金属稳态和解毒中起着重要作用。然而，HIPPs在小麦族物种中的分布、多样性和功能的研究有限。在本研究中，共278个HIPPs从数据库中鉴定的5个小麦族物种，其中13个克隆从簇毛麦。通过系统发育分析将这些基因分为5类。大多数HIPPs有一个HMA结构域，而来自进化枝I的51个有两个，并且所有HIPPs在物种或亚基因组之间具有良好的共线关系。计算机表达谱分析显示，114个小麦HIPPs中有44个主要在根中表达，43个在生物胁迫下上调，29个在干旱或热处理后上调。对克隆的H. villosa表明它们在质膜上表达。HIPP 1-V在H.结果表明，外源镉对小麦种子萌发和根系生长的抑制有明显的恢复作用，而过量表达HIPP 1-V基因的小麦对镉的耐受性增强。本研究提供了小麦族HIPP 1-V基因的全基因组概述，并证明HIPP 1-V正调控普通小麦的镉耐性。
Heavy-metal-associated (HMA) isoprenylated plant proteins (HIPPs) only exist in vascular plants. They play important roles in responses to biotic/abiotic stresses, heavy-metal homeostasis, and detoxification. However, research on the distribution, diversification, and function of HIPPs in Triticeae species is limited. In this study, a total of 278 HIPPs were identified from a database from five Triticeae species, and 13 were cloned from Haynaldia villosa. These genes were classified into five groups by phylogenetic analysis. Most HIPPs had one HMA domain, while 51 from Clade I had two, and all HIPPs had good collinear relationships between species or subgenomes. In silico expression profiling revealed that 44 of the 114 wheat HIPPs were dominantly expressed in roots, 43 were upregulated under biotic stresses, and 29 were upregulated upon drought or heat treatment. Subcellular localization analysis of the cloned HIPPs from H. villosa showed that they were expressed on the plasma membrane. HIPP1-V was upregulated in H. villosa after Cd treatment, and transgenic wheat plants overexpressing HIPP1-V showed enhanced Cd tolerance, as shown by the recovery of seed-germination and root-growth inhibition by supplementary Cd. This research provides a genome-wide overview of the Triticeae HIPP genes and proved that HIPP1-V positively regulates Cd tolerance in common wheat.
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DOI：
10.1073/pnas.1016981108
发表时间：
2011-05-10
影响因子：
11.1
作者：
Cao, Aizhong;Xing, Liping;Chen, Peidu
通讯作者：
Chen, Peidu
新型耐重金属毒性水稻HPP和HIPP基因的鉴定
影响因子：
6.8
作者：
Khan, Irfan Ullah;Rono, Justice Kipkoir;Yang, Zhi Min
通讯作者：
Yang, Zhi Min
影响因子：
7.4
作者：
Galichet, Arnaud;Gruissem, Wilhelm
通讯作者：
Gruissem, Wilhelm
影响因子：
5.2
作者：
Garzon, Teresa;Gunse, Benet;Poschenrieder, Charlotte
通讯作者：
Poschenrieder, Charlotte
影响因子：
56.9
作者：
Avni, Raz;Nave, Moran;Distelfeld, Assaf
通讯作者：
Distelfeld, Assaf