Subtilomycin: a new lantibiotic from Bacillus subtilis strain MMA7 isolated from the marine sponge Haliclona simulans.
Subtilomycin: a new lantibiotic from Bacillus subtilis strain MMA7 isolated from the marine sponge Haliclona simulans.
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DOI：
10.3390/md11061878
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发表时间：
2013-06-03
期刊：
影响因子：
5.4
通讯作者：
Barbosa TM
中科院分区：
文献类型：
--
作者：
Phelan RW;Barret M;Cotter PD;O'Connor PM;Chen R;Morrissey JP;Dobson AD;O'Gara F;Barbosa TM
细菌素作为对抗传染病和耐多药病原体的经典抗生素的替代品，正引起越来越多的关注。从海绵拟海螺中分离出的枯草芽孢杆菌菌株MMA7具有广谱抗菌活性，包括革兰氏阳性和革兰氏阴性病原体，以及几种致病性念珠菌。这种活性部分与一种新发现的抗生素有关，本文将其命名为subtilomycin。提出的生物合成簇由6个基因组成，包括一类抗生素的特征lanb样脱水酶和lanb样环化酶修饰酶的蛋白质编码基因。在枯草芽孢杆菌菌株MMA7中发现了枯草菌素生物合成簇，取代了许多枯草芽孢杆菌分离株中报道的产孢杀死因子（skf）操纵子，并参与了旨在延迟产孢的细菌同类相食行为。从不同的浅水和深水海洋海绵中分离的枯草芽孢杆菌菌株中普遍存在枯草霉素生物合成簇。Subtilomycin具有几种理想的工业和制药物理化学性质，包括在宽pH范围内的活性，耐热性和水溶性。此外，如果枯草芽孢杆菌菌株MMA7作为一种益生菌应用于水产养殖，那么生产低抗生素枯草霉素可能是一个理想的特性。
Bacteriocins are attracting increased attention as an alternative to classic antibiotics in the fight against infectious disease and multidrug resistant pathogens. Bacillus subtilis strain MMA7 isolated from the marine sponge Haliclona simulans displays a broad spectrum antimicrobial activity, which includes Gram-positive and Gram-negative pathogens, as well as several pathogenic Candida species. This activity is in part associated with a newly identified lantibiotic, herein named as subtilomycin. The proposed biosynthetic cluster is composed of six genes, including protein-coding genes for LanB-like dehydratase and LanC-like cyclase modification enzymes, characteristic of the class I lantibiotics. The subtilomycin biosynthetic cluster in B. subtilis strain MMA7 is found in place of the sporulation killing factor (skf) operon, reported in many B. subtilis isolates and involved in a bacterial cannibalistic behaviour intended to delay sporulation. The presence of the subtilomycin biosynthetic cluster appears to be widespread amongst B. subtilis strains isolated from different shallow and deep water marine sponges. Subtilomycin possesses several desirable industrial and pharmaceutical physicochemical properties, including activity over a wide pH range, thermal resistance and water solubility. Additionally, the production of the lantibiotic subtilomycin could be a desirable property should B. subtilis strain MMA7 be employed as a probiotic in aquaculture applications.
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