Adipose-derived and bone marrow mesenchymal stem cells: a donor-matched comparison.
Adipose-derived and bone marrow mesenchymal stem cells: a donor-matched comparison.
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DOI：
10.1186/s13287-018-0914-1
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发表时间：
2018-06-19
影响因子：
7.5
通讯作者：
Idris SB
中科院分区：
文献类型：
--
作者：
Mohamed-Ahmed S;Fristad I;Lie SA;Suliman S;Mustafa K;Vindenes H;Idris SB
脂肪来源的干细胞(ASCs)已被引入作为骨髓间充质干细胞(BMSCs)的替代细胞进行细胞治疗。然而，比较ASCs和BMSCs的不同研究显示出相互矛盾的结果。事实上，从不同个体采集ASCs和BMSCs可能会影响结果，使比较变得困难。因此，本研究旨在对供体相合的ASCs和BMSCs进行鉴定，以探讨其增殖、分化潜能以及供体变异对这些间充质干细胞(MSCs)的可能影响。取9名8~14岁供者的骨髓和脂肪组织标本，分离ASCs和BMSCs，用流式细胞仪检测细胞表面标志物的表达。对增殖至21天的细胞进行了观察。用成骨、成软骨和成脂分化介质诱导多向分化。检测碱性磷酸酶(ALP)活性，免疫荧光染色检测I型胶原形成情况。在体外，使用组织特异性染色和谱系特异性基因表达来研究多效性。此外，在蛋白质水平上对成骨谱系进行了评价。所有供者分离的ASCs和BMSCs具有MSCs的形态和免疫表型特征，表达MSCs标志物，但不表达造血细胞标志物。与BMSCs不同，ASCs高表达CD49d，低表达Stro-1。总的来说，ASCs表现出明显高于BMSCs的增殖和成脂能力，更多的脂泡形成和成脂相关基因的表达。相比之下，BMSCs表现出明显高于ASCs的成骨和软骨形成能力。BMSCs具有较早和较高的碱性磷酸酶活性、钙沉积，以及成骨和软骨形成相关基因和成骨相关蛋白骨桥蛋白的表达。不同供者间ASCs和BMSCs的增殖分化能力差异显著。ASCs和BMSCs显示出组织特异性分化能力，但供者之间存在显著差异。在计划基于干细胞的治疗时，应该考虑到ASCs和BMSCs在特性上的相似和不同。
Adipose-derived stem cells (ASCs) have been introduced as an alternative to bone marrow mesenchymal stem cells (BMSCs) for cell-based therapy. However, different studies comparing ASCs and BMSCs have shown conflicting results. In fact, harvesting ASCs and BMSCs from different individuals might influence the results, making comparison difficult. Therefore, this study aimed to characterize donor-matched ASCs and BMSCs in order to investigate proliferation, differentiation potential and possible effects of donor variation on these mesenchymal stem cells (MSCs). Human bone marrow and adipose tissue samples were obtained from nine donors aged 8–14. ASCs and BMSCs were isolated and characterized based on expression of surface markers using flow cytometry. The proliferation up to 21 days was investigated. Multi-lineage differentiation was induced using osteogenic, chondrogenic and adipogenic differentiation media. Alkaline phosphatase (ALP) activity was monitored and collagen type I formation was evaluated by immunofluorescence staining. In vitro multi-potency was studied using tissue-specific stains and lineage-specific gene expression. In addition, the osteogenic lineage was evaluated at protein level. Isolated ASCs and BMSCs from all donors demonstrated morphologic and immunophenotypic characteristics of MSCs, with expression of MSCs markers and negative expression of hematopoietic markers. Unlike BMSCs, ASCs showed high expression of CD49d and low expression of Stro-1. In general, ASCs showed significantly higher proliferation and adipogenic capacity with more lipid vesicle formation and expression of the adipogenesis-related genes than BMSCs. In contrast, BMSCs showed significantly higher osteogenic and chondrogenic capacity compared to ASCs. BMSCs had earlier and higher ALP activity, calcium deposition, and expression of the osteogenesis- and chondrogenesis-related genes and the osteogenesis-related protein osteopontin. Proliferation and differentiation capacity of ASCs and BMSCs varied significantly among the donors. ASCs and BMSCs showed tissue-specific differentiation abilities, but with significant variation between donors. The similarities and differences in the properties of ASCs and BMSCs should be taken into consideration when planning stem cell-based therapy.
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影响因子：
7.4
作者：
Choudhery MS;Badowski M;Muise A;Pierce J;Harris DT
通讯作者：
Harris DT
影响因子：
3.7
作者：
Beane OS;Fonseca VC;Cooper LL;Koren G;Darling EM
通讯作者：
Darling EM
DOI：
10.1002/stem.2060
发表时间：
2015-09
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Hung BP;Hutton DL;Kozielski KL;Bishop CJ;Naved B;Green JJ;Caplan AI;Gimble JM;Dorafshar AH;Grayson WL
通讯作者：
Grayson WL
影响因子：
3
作者：
Farre-Guasch, Elisabet;Marti-Pages, Carles;Casals, Nuria
通讯作者：
Casals, Nuria
影响因子：
3.6
作者：
Jurgens, Wouter J. F. M.;Oedayrajsingh-Varma, Maikel J.;Helder, Marco N.;ZandiehDoulabi, Behrouz;Scbouten, Tabitha E.;Kuik, Dirk J.;Ritt, Marco J. P. F.;van Milligen, Florine J.
通讯作者：
van Milligen, Florine J.