Precision of and new methods for testing in vitro alloy cytotoxicity.
Precision of and new methods for testing in vitro alloy cytotoxicity.
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体外合金细胞毒性测试的精度和新方法。
DOI：
10.1016/0109-5641(92)90056-i
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hanks,CT
中科院分区：
文献类型：
--
作者：
Wataha,JC;Craig,RG;Hanks,CT
先前的研究利用体外合金细胞毒性测试来评估牙科铸造合金。本研究的目的是：（1）评价先前使用的光密度和目视检查的精密度，（2）评价测量溶解甲瓒染料吸光度的新试验，和（3）检测这些细胞毒性试验之间的相关性。将Balb/c 3 T3细胞以25，000个细胞/cm 2接种于24孔培养盘中的10种牙科铸造合金（6个样品/合金）周围，并孵育72 h。用MTT（3-[4，5-二甲基噻唑-2-基]-2，5-二苯基溴化四唑）琥珀酸盐对细胞进行组织化学染色2小时，然后固定、洗涤并干燥。通过光密度计（OD）扫描、目视评估和DMSO增溶染料的560 nm吸光度测量毒性。没有使用抑制环的测量，因为它们不能提供精确的数据，并且与其他方法的相关性很差。结果用方差分析、Tukey间隔和变异系数（CV）进行分析。MTT需要较短的孵育时间来充分染色，允许单层的溶解，并且比NBT（2，2 ′-二-p-硝基-苯基-5，5 ′-二苯基-3，3 ′-二甲氧基-[3，3 ′-二甲氧基-4，4 ′-联亚苯基]二四唑氯化物）便宜。结果显示，所有三种方法对合金毒性的评级相似（p = 0.05）。由于CV较低，增溶方法最具区分性。密度计和增溶方法之间的相关性极佳（R2= 0.96）。实验间CV一般小于20%，经常小于10%。样本间CV;s通常小于20%。增溶方法的CV始终较低。因此，所有方法均具有良好的重复性和相关性，但增溶法更精确，更具鉴别力。
Previous studies have utilized in vitro alloy cytotoxicity tests to evaluate dental casting alloys. The purposes of this study were to: (1) evaluate the precision of the optical density and visual tests previously used, (2) evaluate a new test measuring absorbance of solubilized formazan dyes, and (3) test the correlation between these tests for cytotoxicity. Balb/c 3T3 cells were plated in 24-well culture trays at 25,000 cells/cm2around ten types of dental casting alloy (six samples/alloy) and incubated for 72 h. Cells were histochemically stained with MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) succinate for 2 h, then fixed, washed, and dried. Toxicity was measured by optical densitometer (OD) scanning, visual assessment, and 560-nm absorbance of DMSO-solubilized dyes. Measurements of rings of inhibition were not used, because they did not provide precise data, and correlated poorly with the other methods. The results were analyzed by ANOVA, Tukey intervals, and coefficients of variation (CV's). MTT required shorter incubation times for adequate staining, allowed for solubilization of the monolayers, and was less expensive than NBT (2,2′-di-p-nitro-phenyl-5,5′-diphenyl-3,3′-dimethoxy-[3,3′-dimethoxy-4,4′-biphenylene] ditetrazolium chloride). Results showed that all three methods ranked alloy toxicities similarly (p = 0.05). The solubilization method was most discriminating due to lower CV's. Correlation between densitometer and solubilization methods was excellent (R2= 0.96). Between-experiment CV's were generally less than 20%, and often less than 10%. Between-sample CV;s were generally less than 20%. CV's were consistently lower for the solubilization method. Thus, all methods were repeatable and correlated well, but the solubilization method was more precise and discriminating.
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影响因子：
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作者：
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DOI：
--
发表时间：
1977
期刊：
Journal of dentistry research
影响因子：
--
作者：
R. Woody;E. F. Huget;J. Horton
通讯作者：
J. Horton
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DOI：
--
发表时间：
1990
期刊：
Journal of Biomedical Materials Research
影响因子：
--
作者：
A. Naji;M. Harmand
通讯作者：
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DOI：
10.1002/jbm.820231002
发表时间：
1989
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
J. Bumgardner;Linda C. Lucas;Arabella B. Tilden
通讯作者：
Arabella B. Tilden
Ag-Pd-Cu 基铸造合金的体外细胞毒性。
DOI：
--
发表时间：
1985
期刊：
Journal of Biomedical Materials Research
影响因子：
--
作者：
L. Niemi;A. Hensten
通讯作者：
A. Hensten