Demonstration of the clathrin- and caveolin-mediated endocytosis at the maternal-fetal barrier in mouse placenta after intravenous administration of gold nanoparticles.
Demonstration of the clathrin- and caveolin-mediated endocytosis at the maternal-fetal barrier in mouse placenta after intravenous administration of gold nanoparticles.
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DOI：
10.1292/jvms.13-0512
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Takano H
中科院分区：
文献类型：
--
作者：
Rattanapinyopituk K;Shimada A;Morita T;Sakurai M;Asano A;Hasegawa T;Inoue K;Takano H
怀孕期间暴露于纳米颗粒是一个公众关注的问题，因为纳米颗粒可能通过胎盘从母亲传给胎儿。本研究的目的是确定金纳米颗粒穿过母胎屏障的可能易位途径以及静脉注射金纳米颗粒对胎盘和胎儿的毒性。在怀孕的第16天和第17天，向怀孕的ICR小鼠静脉注射0.01%的20和50 nm金纳米颗粒溶液。用20和50纳米金纳米颗粒处理的小鼠的胎盘以及母体和胎儿器官没有毒性损伤的迹象。ICP-MS分析表明，在母体肝脏和胎盘中沉积了大量的金，但在胎儿器官中未检测到金。然而，电子显微镜检查显示，金纳米颗粒处理的小鼠母胎屏障中的合胞体滋养层细胞和胎儿内皮细胞的细胞质中的内吞囊泡增加。网格蛋白免疫组织化学和免疫印迹显示，20-和50-nm的金纳米粒子处理的小鼠胎盘组织中的网格蛋白的免疫反应性增加;在合体滋养层细胞和胎儿内皮细胞中观察到网格蛋白免疫阳性。与此相反，小窝蛋白-1免疫阳性观察只在胎儿内皮细胞。这些结果表明，静脉注射金纳米粒子可能上调网格蛋白和小窝蛋白介导的内吞作用在母胎屏障在小鼠胎盘。
Exposure to nanoparticles during pregnancy is a public concern, because nanoparticles may pass from the mother to the fetus across the placenta. The purpose of this study was to determine the possible translocation pathway of gold nanoparticles across the maternal–fetal barrier as well as the toxicity of intravenously administered gold nanoparticles to the placenta and fetus. Pregnant ICR mice were intravenously injected with 0.01% of 20- and 50-nm gold nanoparticle solutions on the 16th and 17th days of gestation. There was no sign of toxic damage to the placentas as well as maternal and fetal organs of the mice treated with 20- and 50-nm gold nanoparticles. ICP-MS analysis demonstrated significant amounts of gold deposited in the maternal livers and placentas, but no detectable level of gold in the fetal organs. However, electron microscopy demonstrated an increase of endocytic vesicles in the cytoplasm of syncytiotrophoblasts and fetal endothelial cells in the maternal–fetal barrier of mice treated with gold nanoparticles. Clathrin immunohistochemistry and immunoblotting showed increased immunoreactivity of clathrin protein in the placental tissues of mice treated with 20- and 50-nm gold nanoparticles; clathrin immunopositivity was observed in syncytiotrophoblasts and fetal endothelial cells. In contrast, caveolin-1 immunopositivity was observed exclusively in the fetal endothelium. These findings suggested that intravenous administration of gold nanoparticles may upregulate clathrin- and caveolin-mediated endocytosis at the maternal–fetal barrier in mouse placenta.
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DOI：
10.1083/jcb.200302028
发表时间：
2003-05-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nabi IR;Le PU
通讯作者：
Le PU
影响因子：
3.8
作者：
Linton, EA;Rodriguez-Linares, B;Redman, CWG
通讯作者：
Redman, CWG
影响因子：
5
作者：
Austin, Carlye A.;Umbreit, Thomas H.;Goering, Peter L.
通讯作者：
Goering, Peter L.
影响因子：
3.8
作者：
Lyden, TW;Anderson, CL;Robinson, JM
通讯作者：
Robinson, JM
影响因子：
64.5
作者：
ROTHBERG, KG;HEUSER, JE;ANDERSON, RGW
通讯作者：
ANDERSON, RGW