piRNAs from Pig Testis Provide Evidence for a Conserved Role of the Piwi Pathway in Post-Transcriptional Gene Regulation in Mammals.
piRNAs from Pig Testis Provide Evidence for a Conserved Role of the Piwi Pathway in Post-Transcriptional Gene Regulation in Mammals.
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DOI：
10.1371/journal.pone.0124860
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Rosenkranz D
中科院分区：
文献类型：
--
作者：
Gebert D;Ketting RF;Zischler H;Rosenkranz D
Piwi相互作用（pi-） rna引导种系表达Piwi蛋白，以抑制转座因子（te）的活性。但值得注意的是，哺乳动物睾丸中大部分粗线素pirna与TEs无关。这就提出了Piwi/piRNA通路是否发挥TE沉默之外的功能的问题。虽然基因来源的pirna被描述了很多次，但由于缺乏反义pirna，可能的基因调控功能受到怀疑。在这里，我们对猪成年睾丸中表达的pirna进行了测序和分析，因为这个分类群拥有一整套哺乳动物Piwi类似物，而它们的精子由于选择性繁殖而具有极端适应性。我们提供了猪piRNA和基因组piRNA集群的详尽表征。此外，我们揭示了正义和反义pirna都源于蛋白质编码基因，同时显示出它们起源于Piwi/ pirna介导的转录后沉默途径（通常称为乒乓周期）的特征。我们进一步发现，猪基因组中大多数已鉴定的piRNA簇跨越蛋白质编码基因或假基因的外显子序列，这揭示了一种针对mRNA的初级反义piRNA可以产生的机制。我们的数据提供了证据，证明来自这些位点的剪接mrna不仅是pirna的靶标，而且还受到乒乓周期加工的影响。最后，我们证明了同源基因在猪、小鼠和人体内被pirna靶向和加工。总之，这强烈表明哺乳动物Piwi/piRNA通路在蛋白质编码基因转录后调控中的保守作用，迄今为止尚未得到太多关注。
Piwi-interacting (pi-) RNAs guide germline-expressed Piwi proteins in order to suppress the activity of transposable elements (TEs). But notably, the majority of pachytene piRNAs in mammalian testes is not related to TEs. This raises the question of whether the Piwi/piRNA pathway exerts functions beyond TE silencing. Although gene-derived piRNAs were described many times, a possible gene-regulatory function was doubted due to the absence of antisense piRNAs. Here we sequenced and analyzed piRNAs expressed in the adult testis of the pig, as this taxon possesses the full set of mammalian Piwi paralogs while their spermatozoa are marked by an extreme fitness due to selective breeding. We provide an exhaustive characterization of porcine piRNAs and genomic piRNA clusters. Moreover, we reveal that both sense and antisense piRNAs derive from protein-coding genes, while exhibiting features that clearly show that they originate from the Piwi/piRNA-mediated post-transcriptional silencing pathway, commonly referred to as ping-pong cycle. We further show that the majority of identified piRNA clusters in the porcine genome spans exonic sequences of protein-coding genes or pseudogenes, which reveals a mechanism by which primary antisense piRNAs directed against mRNA can be generated. Our data provide evidence that spliced mRNAs, derived from such loci, are not only targeted by piRNAs but are also subject to ping-pong cycle processing. Finally, we demonstrate that homologous genes are targeted and processed by piRNAs in pig, mouse and human. Altogether, this strongly suggests a conserved role for the mammalian Piwi/piRNA pathway in post-transcriptional regulation of protein-coding genes, which did not receive much attention so far.
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DOI：
10.1261/rna.045310.114
发表时间：
2014-08
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Hirano T;Iwasaki YW;Lin ZY;Imamura M;Seki NM;Sasaki E;Saito K;Okano H;Siomi MC;Siomi H
通讯作者：
Siomi H
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
影响因子：
10.5
作者：
Babiarz, Joshua E.;Ruby, J. Graham;Blelloch, Robert
通讯作者：
Blelloch, Robert
影响因子：
4.1
作者：
Gebetsberger, Jennifer;Polacek, Norbert
通讯作者：
Polacek, Norbert