A modified method to analyse cell proliferation using EdU labelling in large insect brains.
A modified method to analyse cell proliferation using EdU labelling in large insect brains.
复制标题
DOI：
10.1371/journal.pone.0292009
复制
发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
神经发生的研究对于理解神经系统的进化至关重要。在无脊椎动物中，这一过程已在黑腹果蝇中得到广泛研究，由于先进的遗传工具的可用性，果蝇成为主要模型。然而，昆虫神经系统极其多样化，通过研究一系列分类群，我们可以获得有关神经系统及其发育如何进化的更多信息。昆虫神经系统多样性的高度多样性的一个例子是蘑菇体。蘑菇体在学习和记忆中起着至关重要的作用，并且不同物种的相对大小和它们处理的感官信息类型差异很大。 Heliconiini 蝴蝶为密切相关的分支中的这种多样性提供了有用的快照。在 Heliconiini 属中，相对于大脑的其余部分，Heliconius 属中的蘑菇体比其他密切相关的属大 3-4 倍。这种大小的变化很大程度上是由凯尼恩细胞（形成蘑菇体的内在神经元）数量的增加来解释的。因此，蘑菇体大小的变化是凯尼恩细胞神经发生过程中细胞增殖变化的产物。研究这种变异需要调整标记技术以用于不太常见的研究生物体，因为为常见实验室昆虫开发的方法通常不起作用。在这里，我们提出了一种经过修改的 EdU 染色方案，以检查大脑昆虫的神经发生，使用 Heliconiini 蝴蝶作为我们的主要案例，但也证明了对另一种大脑昆虫蟑螂的适用性。
The study of neurogenesis is critical to understanding of the evolution of nervous systems. Within invertebrates, this process has been extensively studied in Drosophila melanogaster, which is the predominant model thanks to the availability of advanced genetic tools. However, insect nervous systems are extremely diverse, and by studying a range of taxa we can gain additional information about how nervous systems and their development evolve. One example of the high diversity of insect nervous system diversity is provided by the mushroom bodies. Mushroom bodies have critical roles in learning and memory and vary dramatically across species in relative size and the type(s) of sensory information they process. Heliconiini butterflies provide a useful snapshot of this diversity within a closely related clade. Within Heliconiini, the genus Heliconius contains species where mushroom bodies are 3–4 times larger than other closely related genera, relative to the rest of the brain. This variation in size is largely explained by increases in the number of Kenyon cells, the intrinsic neurons which form the mushroom body. Hence, variation in mushroom body size is the product of changes in cell proliferation during Kenyon cell neurogenesis. Studying this variation requires adapting labelling techniques for use in less commonly studied organisms, as methods developed for common laboratory insects often do not work. Here, we present a modified protocol for EdU staining to examine neurogenesis in large-brained insects, using Heliconiini butterflies as our primary case, but also demonstrating applicability to cockroaches, another large-brained insect.
登录
查看更多内容
影响因子：
2.6
作者：
通讯作者：
--
影响因子：
56.9
作者：
Gould, E;Reeves, AJ;Gross, CG
通讯作者：
Gross, CG
影响因子：
2.5
作者：
FAHRBACH, SE;STRANDE, JL;ROBINSON, GE
通讯作者：
ROBINSON, GE
影响因子：
--
作者：
Hartenstein, Volker;Wodarz, Andreas
通讯作者：
Wodarz, Andreas
DOI：
10.1073/pnas.0508430102
发表时间：
2005-11-29
影响因子：
11.1
作者：
Farris, SM;Roberts, NS
通讯作者：
Roberts, NS