sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination.
sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination.
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DOI：
10.1371/journal.pgen.1009878
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发表时间：
2021-10
期刊：
影响因子：
4.5
通讯作者：
Lim HM
中科院分区：
文献类型：
--
作者：
Jeon HJ;Lee Y;N MPA;Wang X;Chattoraj DK;Lim HM
在细菌中，小的非编码rna （sRNAs）结合目标mrna并调节其翻译和/或稳定性。在大肠杆菌的多顺反子galETKM操纵子中，Spot 42 sRNA与操纵子转录物结合导致galET mRNA的产生。这一监管的机制尚不清楚。我们发现，在galK基因开始的sRNA-mRNA碱基配对导致galK内的转录终止和转录物切割，并产生具有两种不同3 ' -OH末端的galET mrna。转录终止需要Rho，转录物切割需要内切酶RNase e。产生两种galET所需的sRNA-mRNA碱基配对片段不同，表明这两种事件对序列的要求不同。在mRNA中使用两个靶标，每个靶标都会导致不同的结果，这似乎是sRNA的一种新的作用模式。考虑到潜在的sRNA靶点在顺反子连接处的普遍存在，通过本文报道的机制产生新的mRNA物种可能是细菌基因调控的一种普遍模式。sRNAs是所有生命形式中基因表达的调节器。在大肠杆菌等细菌中，sRNAs碱基与mRNA配对，这可能会产生许多后果，如Rho的过早转录终止和核糖核酸内切酶RNase e对mRNA的降解。在这里，我们发现这两个过程可以发生在同一sRNA-mRNA对上，并产生更短但稳定的mRNA，这可能具有功能意义。因此，在sRNA对mRNA的调控中，转录物切割可能是已知的转录终止之外产生新的mRNA物种的另一种机制。这两种机制之间的选择取决于与sRNA碱基配对发生在mRNA上的哪个位置。
In bacteria, small non-coding RNAs (sRNAs) bind to target mRNAs and regulate their translation and/or stability. In the polycistronic galETKM operon of Escherichia coli, binding of the Spot 42 sRNA to the operon transcript leads to the generation of galET mRNA. The mechanism of this regulation has remained unclear. We show that sRNA-mRNA base pairing at the beginning of the galK gene leads to both transcription termination and transcript cleavage within galK, and generates galET mRNAs with two different 3’-OH ends. Transcription termination requires Rho, and transcript cleavage requires the endonuclease RNase E. The sRNA-mRNA base-paired segments required for generating the two galET species are different, indicating different sequence requirements for the two events. The use of two targets in an mRNA, each of which causes a different outcome, appears to be a novel mode of action for a sRNA. Considering the prevalence of potential sRNA targets at cistron junctions, the generation of new mRNA species by the mechanisms reported here might be a widespread mode of bacterial gene regulation. sRNAs are regulators of gene expression in all forms of life. In bacteria such as E. coli, sRNAs base-pair with mRNA, which can have many consequences such as premature transcription termination by Rho and degradation of mRNA by the endoribonuclease RNase E. Here we show that the two processes can occur on the same sRNA-mRNA pair and produce shorter but stable mRNA that can be of functional significance. Thus, in sRNA regulation of mRNA, transcript cleavage can be an additional mechanism to well-known transcription termination to generate new mRNA species. The choice between the mechanisms is dictated by where on the mRNA the base pairing with sRNA takes place.
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影响因子：
14.9
作者：
Kalvari I;Argasinska J;Quinones-Olvera N;Nawrocki EP;Rivas E;Eddy SR;Bateman A;Finn RD;Petrov AI
通讯作者：
Petrov AI
影响因子：
64.5
作者：
CHEN, CYA;BEATTY, JT;BELASCO, JG
通讯作者：
BELASCO, JG
DOI：
10.1038/newbio241260a0
发表时间：
1973-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
DECROMBR.B;ADHYA, S;PASTAN, I
通讯作者：
PASTAN, I
DOI：
10.1126/science.1251871
发表时间：
2014-05-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Larson MH;Mooney RA;Peters JM;Windgassen T;Nayak D;Gross CA;Block SM;Greenleaf WJ;Landick R;Weissman JS
通讯作者：
Weissman JS
影响因子：
16
作者：
Bandyra, Katarzyna J.;Said, Nelly;Pfeiffer, Verena;Gorna, Maria W.;Vogel, Joerg;Luisi, Ben F.
通讯作者：
Luisi, Ben F.