Seasonal variation in the biocontrol efficiency of bacterial wilt is driven by temperature-mediated changes in bacterial competitive interactions.
Seasonal variation in the biocontrol efficiency of bacterial wilt is driven by temperature-mediated changes in bacterial competitive interactions.
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青枯病生物防治效率的季节性变化是由温度介导的细菌竞争性相互作用的变化驱动的
DOI：
10.1111/1365-2664.12873
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Friman VP
中科院分区：
文献类型：
--
作者：
Wei Z;Huang J;Yang T;Jousset A;Xu Y;Shen Q;Friman VP
基于微生物的生物防治应用在未来有可能成为控制植物病原体疾病爆发的有效方法。然而，它们的效率仍然非常多变，这可能是由于它们对非生物环境条件的敏感性。在此，我们评估了环境温度变化与中国不同番茄种植季节内生细菌生物防治剂——尖克Ralstonia pickettii抑制番茄青枯病病原菌能力的关系。我们发现，当季节平均温度在20℃左右时，对病原菌的抑制作用最高，随着作物季节平均温度的升高而迅速下降。有趣的是，低水平的疾病发病率与低病原体或高生物防治剂绝对密度无关。相反，生物防治与病原体密度比是不同作物季节间疾病发病率水平的更重要预测指标。为了从机制上理解这一点，我们在体外自然发生的温度梯度上测量了生物防治剂和病原体之间的生长和竞争强度。我们发现，生物防治菌株在低温范围内生长较快，病原菌在高温范围内生长较快，并且与田间实验相似，病原菌抑制在20℃时达到峰值。总之，我们的研究结果表明，温度介导的细菌竞争强度的变化可能解释了中国不同作物季节间茄青霉不同的生物防治效果。合成与应用。我们的研究结果表明，非生物环境条件，如温度，可以影响生物防治应用的效果。因此，为了在未来开发更一致的生物防治应用，我们可能需要找到并分离出无论环境条件如何变化都能保持其功能的菌株。我们的研究结果表明，非生物环境条件，如温度，可以影响生物防治应用的效果。因此，为了在未来开发更一致的生物防治应用，我们可能需要找到并分离出无论环境条件如何变化都能保持其功能的菌株。
Microbe‐based biocontrol applications hold the potential to become an efficient way to control plant pathogen disease outbreaks in the future. However, their efficiency is still very variable, which could be due to their sensitivity to the abiotic environmental conditions. Here, we assessed how environmental temperature variation correlates with ability of Ralstonia pickettii, an endophytic bacterial biocontrol agent, to suppress the Ralstonia solanacearum pathogen during different tomato crop seasons in China. We found that suppression of the pathogen was highest when the seasonal mean temperatures were around 20 °C and rapidly decreased with increasing mean crop season temperatures. Interestingly, low levels of disease incidence did not correlate with low pathogen or high biocontrol agent absolute densities. Instead, the biocontrol to pathogen density ratio was a more important predictor of disease incidence levels between different crop seasons. To understand this mechanistically, we measured the growth and strength of competition between the biocontrol agent and the pathogen over a naturally occurring temperature gradient in vitro. We found that the biocontrol strain grew relatively faster at low temperature ranges, and the pathogen at high temperature ranges, and that similar to field experiments, pathogen suppression peaked at 20 °C. Together, our results suggest that temperature‐mediated changes in the strength of bacterial competition could potentially explain the variable R. solanacearum biocontrol outcomes between different crop seasons in China. Synthesis and applications. Our results suggest that abiotic environmental conditions, such as temperature, can affect the efficacy of biocontrol applications. Thus, in order to develop more consistent biocontrol applications in the future, we might need to find and isolate bacterial strains that can retain their functionality regardless of the changing environmental conditions. Our results suggest that abiotic environmental conditions, such as temperature, can affect the efficacy of biocontrol applications. Thus, in order to develop more consistent biocontrol applications in the future, we might need to find and isolate bacterial strains that can retain their functionality regardless of the changing environmental conditions.
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