Analytic Microdensitometer for Quantitative Cytochemistry

用于定量细胞化学的分析微密度计

• 批准号: 8604786
• 负责人: Lawrence Zoller
• 金额: $ 6.56万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-15 至 1989-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8604786&HistoricalAwards=false
• 关键词: Analytic; Microdensitometer; Quantitative; Cytochemistry

An analytical microdensitometer will be acquired to quantitatively measure a variety of reaction products visualized in microscopic sections of biological tissues. The machine is to be applied to a number of projects: 1) Changes in enzyme levels in ovarian cells as a result of exposure to tetrahydrocannabinol: 2) The process of degeneration in pericytes of diabetics; 3) Transmitter related enzymes in the central nervous system of monkeys; 4) The relationship of behavioral change to lesion-induced alterations in transmitter related enzymes in the central nervous system of monkeys; 5) Changes in the superior cervical ganglion as a result of exposure to testosterone or estradiol; and 6) Changes in the motor unit as a result of age or lesion. One of the exciting developments in instrumentation for biological research is the ability to couple microscopes to computers to allow easy quantitative analysis of microscopic images. All projects are critically dependent upon the densitometer as an investigative tool and will exploit the machine's ability to measure product on sections at various wavelengths with a great degree of accuracy and reproducibility. The availability of this instrument will allow several excellent investigators to greatly strengthen their research programs.

将购置一台分析显微密度计， 定量测量可视化的各种反应产物 在生物组织的显微切片中。 本机适用于多个项目：1）改变 卵巢细胞中的酶水平， 四氢大麻酚：2）周细胞的变性过程 糖尿病患者; 3）中枢递质相关酶 4）行为与神经系统的关系 病变诱导的递质相关改变 猴子中枢神经系统中的酶; 5） 上级颈神经节由于暴露于 睾酮或雌二醇;和6）运动单位的变化， 年龄或病变的结果。 仪器仪表的一个令人兴奋的发展是， 生物学研究是将显微镜与 计算机可以方便地定量分析微观 图像. 所有项目都严重依赖于密度计， 调查工具，并将利用机器的能力， 以良好的测量性能在不同波长的切片上测量产品 准确度和再现性。 这个的可用性 仪器将允许几个优秀的调查人员大大 加强他们的研究计划。