Development of Separation and Purification Processes: Chromatographic Scale-Up

生产高容量的生物活性化合物在生物技术中至关重要 动物健康、食品加工和人类健康需要大量的 生物反应器体积。 因此，下游加工的规模扩大 步骤成为越来越重要的因素。 实例包括 胰岛素、人和动物生长激素以及选定类别的 抗生素 生物分离的关键领域包括吸附和 色谱分离和这些形成了本研究的重点。 基础数据库和放大协议， 发酵衍生分子的色谱纯化是 通常相对于其它生物分离方法缺乏。 目标 本研究计划的主要目的是扩展过程的知识基础， 色谱和吸附，以促进其大规模使用。

Production of high volume biologically active compounds imortant in animal health, food processing, and human health require large bioractor ovolumes. Consequently, scale-up of downstream processing steps become an increasingly important factor. Examples include insulin, human and animal growth hormones, and selected classes of antibiotics. Key areas in bioseparations include adsorptive and chromatiographic separations and these form the focus of this research. Fundamental data bases and scale-up protocols for adsorptive and chromatographic purification of fermentation-derived molecules are generally lacking relative to other bioseparation processes. The goal of this research program is to expand the knowledge base of process chromatography and adsorption to promote its use on a large scale.