Development of Separation and Purification Processes: Chromatographic Scale-Up

大量生产对动物健康、食品加工和人类健康有重要影响的生物活性化合物需要大量的生物反应器。因此，下游加工步骤的放大成为一个日益重要的因素。例子包括胰岛素、人类和动物生长激素，以及特定类别的抗生素。生物分离的关键领域包括吸附分离和色谱分离，这些都是本研究的重点。与其他生物分离过程相比，用于吸附和层析纯化发酵衍生分子的基础数据库和放大协议通常是缺乏的。该研究项目的目标是扩大过程色谱和吸附的知识库，促进其大规模应用。

Production of high volume biologically active compounds imortant in animal health, food processing, and human health require large bioractor ovolumes. Consequently, scale-up of downstream processing steps become an increasingly important factor. Examples include insulin, human and animal growth hormones, and selected classes of antibiotics. Key areas in bioseparations include adsorptive and chromatiographic separations and these form the focus of this research. Fundamental data bases and scale-up protocols for adsorptive and chromatographic purification of fermentation-derived molecules are generally lacking relative to other bioseparation processes. The goal of this research program is to expand the knowledge base of process chromatography and adsorption to promote its use on a large scale.