Engineering Research Equipment: Quantitative Image Process-ing System for Darkfield Microscope

工程研究设备：暗视野显微镜定量图像处理系统

• 批准号: 8705121
• 负责人: Michael Ladisch
• 金额: $ 6.04万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8705121&HistoricalAwards=false
• 关键词: Engineering; Research; Equipment; Quantitative; Image

Progress has been made on analyzing micelle coagulation in a biological system (milk). Using a Zeiss IM, darkfield microscope with TV monitor, camera, and microflash, particles as small as 0.095 micron were detected when the proteolytic enzyme rennet was added to diluted milk, and the time course of coagulation was observed. For this project, Zeiss IBAS image processing system will be used to attach to the darkfield microscope already in place in his laboratory. The system will allow particle sizes to be determined, enhancement of images from the darkfield microscope (increasing resolution), and analysis of these images. After image enhancement, the geometric shape factors for the model simulation can be calculated. Comparison of these shape factors for the actual flocs and the simulated flocs will test the applicability of theoretical models.

生物反应器中胶束凝聚的研究取得了进展， 牛奶（milk） 使用带有电视监视器的蔡司IM暗场显微镜， 相机和微型闪光灯，小至0.095微米的颗粒， 当将蛋白水解酶凝乳酶加入稀释的牛奶中时检测到， 并观察凝血时间过程。 对于这个项目， 蔡司IBAS图像处理系统将用于附加到 暗视野显微镜已经在他的实验室。 系统 将允许确定颗粒大小，增强图像， 暗场显微镜（提高分辨率），并分析这些 图像. 在图像增强之后， 模型模拟可以计算。 这些形状因子的比较 对实际絮体和模拟絮体进行测试， 理论模型的适用性。