Engineering Research Equipment: Quantitative Image Process-ing System for Darkfield Microscope

在分析生物体系(牛奶)中胶束凝结方面取得了进展。利用蔡司IM、配有电视监视器、摄像机和微闪光灯的暗场显微镜，在稀释的牛奶中加入蛋白水解酶凝乳酶，检测到细小为0.095微米的颗粒，并观察了凝乳的时间过程。在这个项目中，蔡司IBAS图像处理系统将被用来安装在他实验室里已经就位的暗场显微镜上。该系统将允许确定颗粒大小，增强来自暗视野显微镜的图像(提高分辨率)，并分析这些图像。经过图像增强后，可以计算出模型仿真所需的几何形状因子。将实际絮体和模拟絮体的这些形状因子进行比较，将检验理论模型的适用性。

Progress has been made on analyzing micelle coagulation in a biological system (milk). Using a Zeiss IM, darkfield microscope with TV monitor, camera, and microflash, particles as small as 0.095 micron were detected when the proteolytic enzyme rennet was added to diluted milk, and the time course of coagulation was observed. For this project, Zeiss IBAS image processing system will be used to attach to the darkfield microscope already in place in his laboratory. The system will allow particle sizes to be determined, enhancement of images from the darkfield microscope (increasing resolution), and analysis of these images. After image enhancement, the geometric shape factors for the model simulation can be calculated. Comparison of these shape factors for the actual flocs and the simulated flocs will test the applicability of theoretical models.