Construction of an Indicator Gene Vector for Obtaining On-Line Protein Translation and Accumulation Rate Data

用于获取在线蛋白质翻译和积累率数据的指示基因载体的构建

• 批准号: 8618134
• 负责人: Michael Flickinger
• 金额: $ 3万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-15 至 1988-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8618134&HistoricalAwards=false
• 关键词: Construction; Indicator; Gene; Vector; Obtaining

This project involves the development of a method for monitoring the extent of specifically induced protein biosynthesis by genetically engineered microorganisms. The approach induces the formation of another enzyme (at a related but must lower rate) which can be easily measured by spectrophotometric techniques. This project has the potential to provide a general method for monitoring the productivity of genetically engineered microorganisms.

该项目涉及开发一种方法，用于监测基因工程微生物特异性诱导的蛋白质生物合成的程度。 该方法诱导另一种酶的形成（以相关但必须较低的速率），可以通过分光光度技术轻松测量。 该项目有可能提供一种监测基因工程微生物生产力的通用方法。