Enhanced Gene Expression in Immobilized Whole-Cell Biocatalysis

9424063 Flickinger这个项目正在研究固定细胞的聚合物技术以及受限细胞在饥饿条件下的行为。固定化系统由三层组成：无孔载体；细胞和聚合物；以及薄覆盖的皮肤。将对制备和储存后的细胞活力以及不同基因的表达、消息的稳定性等进行研究。基质的形态也将被表征，并用于改善生物结果。这项研究的一个主要目标是最大化固定化细胞的活性。此外，还将检查通过添加营养物质使细胞周期性复苏的情况。***

9424063 Flickinger This project is on research to study the polymer technology of immobilizing cells as well as the behavior of confined cells under starvation conditions. The immobilization system consists of three layers: a non porous support; cells and polymer; and a thin covering skin. Cell viability after preparation and storage, as well as the expression of different genes, message stability, etc. will be investigated. The morphology of the matrix will also be characterized and used to improve the biological outcome. A major goal of the research is to maximize the viability of the immobilized cells. Also, the periodic revival of the cells by nutrient addition will be examined. ***