Enhanced Gene Expression in Immobilized Whole-Cell Biocatalysis

固定化全细胞生物催化中基因表达的增强

• 批准号: 9424063
• 负责人: Michael Flickinger
• 金额: $ 32.75万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9424063&HistoricalAwards=false
• 关键词: Enhanced; Gene; Expression; Immobilized; Whole

9424063 Flickinger This project is on research to study the polymer technology of immobilizing cells as well as the behavior of confined cells under starvation conditions. The immobilization system consists of three layers: a non porous support; cells and polymer; and a thin covering skin. Cell viability after preparation and storage, as well as the expression of different genes, message stability, etc. will be investigated. The morphology of the matrix will also be characterized and used to improve the biological outcome. A major goal of the research is to maximize the viability of the immobilized cells. Also, the periodic revival of the cells by nutrient addition will be examined. ***

9424063 Flickinger 该项目旨在研究固定细胞的聚合物技术以及限制细胞在饥饿条件下的行为。 固定系统由三层组成：无孔支撑；细胞和聚合物；和一层薄薄的覆盖皮肤。 将研究制备和储存后的细胞活力，以及不同基因的表达、信息稳定性等。 基质的形态也将被表征并用于改善生物学结果。 该研究的一个主要目标是最大限度地提高固定细胞的活力。 此外，还将检查通过添加营养物质而使细胞周期性再生的情况。 ***