U.S.-Netherlands Cooperative Research: Cell Biology of Receptor Mediated Endocytosis

美国-荷兰合作研究：受体介导的内吞作用的细胞生物学

• 批准号: 8619966
• 负责人: Alan Schwartz
• 金额: $ 0.84万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8619966&HistoricalAwards=false
• 关键词: U.S.; Netherlands; Cooperative; Research; Cell

This is a three-year cooperative research project in cellular biology between Professor Alan L. Schwartz, Professor of Pediatrics and Professor of Pharmacology, Washington University School of Medicine, St. Louis, MO, and Professors H. J. Geuze and G. J. A. M. Strous, Center for Electron Microscopy, University of Utrecht, The Netherlands. The cooperating scientists and members of their research groups will continue their efforts to elucidate the cell biology of receptor mediated endocytosis (RME) and to define this process in both biochemical and morphological terms. The investigators will use the hepatic asialoglycoprotein receptor as their model for the study of RME. Receptor mediated endocytosis is a process by which extracellular macromolecules and particles gain entry to the intracellular environment. The process is responsible for the cellular uptake and delivery of a broad class of substances which include essential cell nutrients (e.g. iron, Vitamin B-12), toxins (e.g. diphtheria toxin), cell growth factors/hormones (e.g. insulin), viruses (e.g. influenza), and proteins undergoing clearance from the circulation (e.g. asialoglycoprotein). This cooperative study is expected to lead to an increased understanding of the molecular basis of cell-cell communication and the transfer of biochemical information from outside of a cell to within it. Such an understanding of the basic biology of RME would have direct applicability to the elucidation of cellular processes in normal cells, and in addition provide a background on which to base new approaches to directed drug therapy. The approach here will combine a biochemical and molecular biological aspect (Professor Schwartz's expertise) with ultrastructural immunocytochemistry (the expertise of the Dutch investigators). Professor Geuze's laboratory in Utrecht is equipped with several state-of-the-art electron microscopes and ultra-cryo microtomes which will be utilized in this study. Funds from this award will support necessary transportation and subsistence expenses for the U.S. principal investigator and members of his research group. The government of The Netherlands, through the University of Utrecht, is also providing support for the project.

这是一项为期三年的细胞生物学合作研究项目，由密苏里州圣路易斯市华盛顿大学医学院儿科学教授兼药理学教授艾伦·L·施瓦茨教授与荷兰乌得勒支大学电子显微镜中心的H·J·格兹和G·J·A·M·斯特鲁斯教授共同完成。合作的科学家和他们的研究小组成员将继续努力阐明受体介导的内吞作用(RME)的细胞生物学，并从生化和形态两个方面定义这一过程。研究人员将使用肝脏去唾液酸糖蛋白受体作为他们研究RME的模型。受体介导的内吞作用是细胞外大分子和颗粒进入细胞内环境的过程。这一过程负责细胞对多种物质的摄取和传递，这些物质包括基本的细胞营养物质(例如铁、维生素B-12)、毒素(例如白喉毒素)、细胞生长因子/激素(例如胰岛素)、病毒(例如流感)和从循环中清除的蛋白质(例如去唾液糖蛋白)。这项合作研究有望增进对细胞间通讯的分子基础的理解，以及生化信息从细胞外转移到细胞内的过程。这种对RME基本生物学的了解将直接适用于阐明正常细胞的细胞过程，此外还为定向药物治疗的新方法提供了基础。这里的方法将结合生化和分子生物学方面(施瓦茨教授的专业知识)和超微结构免疫细胞化学(荷兰研究人员的专业知识)。格兹教授在乌得勒支的实验室配备了几台最先进的电子显微镜和超低温显微镜，将在本研究中使用。这项奖励的资金将支持美国首席研究员及其研究小组成员必要的交通和生活费用。荷兰政府也通过乌得勒支大学为该项目提供支持。