Acquisition of an Open-Flow Microcalorimetry and Respirometry System

本提案要求为购买一套开放流动微热量-呼吸测量系统提供资金。该仪器将实现目前无法获得的分析能力，并将促进由五个主要用户和三个次要用户指导的现有项目的扩展和增强。它将支持以下研究：(1)无脊椎动物低代谢状态的机制分析，(2)骨骼肌糖生成的调节，(3)浮游无脊椎动物的代谢适应，(4)同类植物耐洪水的生理基础，以及(5)土壤微生物基于硝基酚的代谢。除了量化各自组织/生物体的总代谢率外，微热-呼吸系统还将允许厌氧成分，监测进入和存在于应激环境条件中的代谢补偿，并分析不同底物对总代谢率的相对贡献。

This proposal requests funds for the purchase of an open-flow microcalorimetry-respirometry system. This instrumentation will enable analytical capabilities not presently available and will foster the expansion and enhancement of existing programs directed by five primary and three secondary users. It will support research on (l) mechanistic analyses of hypometabolic states in invertebrates, (2) regulation of glucoeogenesis in skeletal muscle, (3) metabolic adaptations of planktonic invertebrates, (4) physiological basis for flood-tolerance among congeneric plant species, and (5) nitrophenol-based metabolism of soil microbes. In addition to quantifying total metabolic rates of the respective tissues/organisms, the microcalorimetry- respirometry system will allow anaerobic components, monitoring of metabolic compensation during entry and exist from stressful environmental conditions, and analysis of the relative contributions of different substrates to total metabolic rate.