Electron Transfer in Cytochromes: Controlling Factors

细胞色素中的电子转移：控制因素

• 批准号: 8707447
• 负责人: Dabney Dixon
• 金额: $ 5万
• 依托单位: Georgia State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1989-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8707447&HistoricalAwards=false
• 关键词: Electron; Transfer; Cytochromes; Controlling; Factors

This award is a Career Advancement Award under the Research Opportunities for Women Program. The award will be used to gain mastery of biochemical techniques necessary for protein manipulation in a project concerned with the mechanisms of biological electron transfer. Electron transfer is one of the most fundamental biological processes. It is an integral part of many metabolic pathways and plays a vital role in the utilization of oxygen by biological systems. The focus of the research project is electron transfer between cytochromes, a family of metalloproteins which undergo electron transfer during the utilization of oxygen. The study will focus on the three factors which are expected to be the primary determinants of cytochrome electron transfer: electrostatics, the distance between the two hemes, and protein motion. Electron self-exchange rate constants will be measured for: a) cytochromes from various species, b) cytochromes with derivatized residues, c) cytochromes in which the native heme has been replaced with a synthetic heme, d) cytochromes in which there is a break in the polypeptide chain, and e) cytochromes synthesized via site-directed mutagenesis. Self-exchange rate constants will be measured by NMR spectroscopy.

该奖项是女性研究机会计划下的职业发展奖。该奖项将用于在与生物电子转移机制有关的项目中掌握蛋白质操作所需的生化技术。电子转移是最基本的生物过程之一。它是许多代谢途径的组成部分，在生物系统利用氧气方面发挥着至关重要的作用。 该研究项目的重点是细胞色素之间的电子转移，细胞色素是一种金属蛋白家族，在利用氧气时会发生电子转移。该研究将重点关注预计将成为细胞色素电子转移主要决定因素的三个因素：静电、两个血红素之间的距离和蛋白质运动。 将测量以下物质的电子自交换速率常数：a) 来自不同物种的细胞色素，b) 具有衍生残基的细胞色素，c) 其中天然血红素已被合成血红素取代的细胞色素，d) 其中多肽链断裂的细胞色素，以及 e) 通过定点诱变合成的细胞色素。 自交换速率常数将通过核磁共振波谱测量。