An Investigation of the Cellular Events that Initiate and Control the Deposition of Patterned Walls in Plant Cells

植物细胞中启动和控制图案壁沉积的细胞事件的研究

• 批准号: 8710243
• 负责人: Candace Haigler
• 金额: $ 24.8万
• 依托单位: Texas Tech University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-10-01 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8710243&HistoricalAwards=false
• 关键词: Investigation; Cellular; Events; Initiate; Control

This research will exploit a unique developmental system (in which mesophyll cells of >Zinnia> >elegans> differentiate in suspension culture to form tracheary elements with distinctly patterned secondary walls) in order to obtain a more integrated view of how higher plant cells initiate and control the deposition of a patterned wall. Five major areas of reserach will be conducted to meet this goal: (1) Light and electron microscopy to clarify the timing and cytology of differentiation; (2) Investigation of the role of microtubules; (3) Investigation of the role of microfilaments; (4) Investigation of the role of the Golgi apparatus and the functional life of rosettes (particles associated with cellulose synthesis) in the plasma membrane; and (5) Investigation of calcium.mediated events critical to the initiation and continuation of differentiation. Light and electron microscopy (coupled with immunochemical and cytochemical probes) will be used to monitor changes in differentiation.related cellular processes before and after application of antagonistic drugs. The results of this study will increase our knowledge of how diverse cellular features function together in the control of a well.defined developmental event. They will also enhance our understanding of the control of plant morphogenesis, which is partly determined by the pattern of microfibrils in cell walls.

这项研究将利用一种独特的发育系统（其中>百日草>>线虫>的叶肉细胞在悬浮培养中分化形成具有明显图案化次生壁的气管元件），以获得关于高等植物细胞如何启动和控制图案化壁沉积的更全面的观点。 为了实现这一目标，将进行五个主要研究领域：（1）光学和电子显微镜以阐明分化的时间和细胞学； (2)微管作用的研究； (3)微丝作用的考察； (4) 研究高尔基体的作用和质膜中玫瑰花结（与纤维素合成相关的颗粒）的功能寿命； (5)研究对分化的起始和持续至关重要的钙介导事件。 光学和电子显微镜（结合免疫化学和细胞化学探针）将用于监测应用拮抗药物前后分化相关细胞过程的变化。 这项研究的结果将增加我们对不同细胞特征如何在控制明确的发育事件中共同发挥作用的了解。 它们还将增强我们对植物形态发生控制的理解，植物形态发生部分是由细胞壁中微原纤维的模式决定的。