An Investigation of the Cellular Events that Initiate and Control the Deposition of Patterned Walls in Plant Cells

这项研究将利用一种独特的发育系统(在悬浮培养中，百日草的叶肉细胞分化形成具有明显图案的次生壁的管状分子)，以获得更全面的观点，了解高等植物细胞如何启动和控制图案壁的沉积。为了实现这一目标，将进行五个主要领域的研究：(1)光学和电子显微镜以阐明分化的时机和细胞学；(2)微管的作用的调查；(3)微丝的作用的调查；(4)研究高尔基体的作用和花环(与纤维素合成有关的颗粒)在质膜中的功能寿命；(5)研究钙介导的对分化的启动和继续至关重要的事件。光镜和电子显微镜(结合免疫化学和细胞化学探针)将被用来监测使用拮抗药物前后分化相关细胞过程的变化。这项研究的结果将增加我们对不同的细胞功能如何在控制明确的发育事件中共同发挥作用的了解。它们还将加强我们对植物形态发生控制的理解，这在一定程度上是由细胞壁中的微纤维模式决定的。

This research will exploit a unique developmental system (in which mesophyll cells of >Zinnia> >elegans> differentiate in suspension culture to form tracheary elements with distinctly patterned secondary walls) in order to obtain a more integrated view of how higher plant cells initiate and control the deposition of a patterned wall. Five major areas of reserach will be conducted to meet this goal: (1) Light and electron microscopy to clarify the timing and cytology of differentiation; (2) Investigation of the role of microtubules; (3) Investigation of the role of microfilaments; (4) Investigation of the role of the Golgi apparatus and the functional life of rosettes (particles associated with cellulose synthesis) in the plasma membrane; and (5) Investigation of calcium.mediated events critical to the initiation and continuation of differentiation. Light and electron microscopy (coupled with immunochemical and cytochemical probes) will be used to monitor changes in differentiation.related cellular processes before and after application of antagonistic drugs. The results of this study will increase our knowledge of how diverse cellular features function together in the control of a well.defined developmental event. They will also enhance our understanding of the control of plant morphogenesis, which is partly determined by the pattern of microfibrils in cell walls.