An Investigation of the Cellular Events that Control the Deposition of Patterned Walls in Plant Cells

控制植物细胞图案壁沉积的细胞事件的研究

• 批准号: 9106704
• 负责人: Candace Haigler
• 金额: $ 25.5万
• 依托单位: Texas Tech University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9106704&HistoricalAwards=false
• 关键词: Investigation; Cellular; Events; Control; Deposition

This research will exploit a unique developmental system in which isolated mesophyll cells differentiate synchronously to form tracheary elements. Tracheary elements are characterized by distinctly patterned secondary walls, but it is not known how wall deposition and patterning are controlled. Prior results demonstrated requirements for calcium sequestration and transport before and during wall deposition, thereby providing the first evidence for a regulatory ion the might coordinate diverse events in cell wall deposition. These and other results will be extended in three directions: (1) Determination of which events in wall deposition are regulated by calcium; (2) Determination of how wall patterning is controlled, particularly in regard to self-determining interactions of wall components; and (3) Characterization of a plasma membrane associated protein associated with cell wall patterning. The research will use modern cytochemical, spectroscopic, and molecular biological techniques for molecular dissection of cell wall structure and control of patterning. It will represent the first attempt to obtain a molecular understanding of the temporal and spatial control of synthesis of cell wall tracheary elements, which are classical specimens in plant developmental biology and have a significant role in plant adaptation.

这项研究将利用一个独特的发展系统， 离体叶肉细胞同步分化形成 维管分子 导管分子的特征是 明显图案化的次生壁，但不知道如何 控制壁沉积和图案化。 先前结果 已证实的钙螯合和运输要求 在壁沉积之前和期间，从而提供第一 有证据表明一个调节离子可能协调不同的事件 细胞壁沉积。 这些和其他结果将是 三个方向：（1）确定哪些事件 细胞壁中钙的沉积受钙的调节;（2）测定细胞壁中钙的含量， 如何控制壁图案，特别是关于 墙体构件的自决定相互作用;以及（3） 一种质膜相关蛋白的鉴定 与细胞壁图案有关。 研究将使用 现代细胞化学、光谱学和分子生物学 细胞壁结构的分子解剖技术， 控制模式。 这将是第一次尝试， 从分子水平上理解 控制细胞壁导管分子的合成， 植物发育生物学中的经典标本， 在植物适应中的重要作用。