Development of a Facility for Image Analysis of Helical Polymers

螺旋聚合物图像分析设备的开发

• 批准号: 8712075
• 负责人: Edward Egelman
• 金额: $ 18.68万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-15 至 1989-08-01
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8712075&HistoricalAwards=false
• 关键词: Development; Facility; Image; Analysis; Helical

The principal investigator proposes to develop a workstation, operating software, and methods that will allow researchers to analyze both the structure and dynamics of disordered helical polymers. The facility will enable electron micrographs to be rapidly digitized and stored in a computer so that the images may be processed at near real-time speeds, with all processing interactive via a raster graphic display. The software being developed will allow for rapid analysis of hundreds of images. Assemblage of large statistical data bases of images, and their associated parameters will allow for the averaging of subsets of images. Dynamical models of these protein polymers can then be generated from the different conformations. The disorder, which is presently an obstacle to structure determination, will be exploited to reveal how these molecules flucturate both randomly and as part of enzymatic cycles. The study of biological structures should be one of the major beneficiaries of this technology.

主要研究者建议开发一个工作站， 操作软件和方法，使研究人员能够 分析无序螺旋的结构和动力学 聚合物 该设施将使电子显微照片， 快速数字化并存储在计算机中， 以接近实时的速度处理，所有处理 通过光栅图形显示进行交互。 述软件被 它将允许快速分析数百个图像。 图像的大型统计数据库的集合及其 相关联的参数将允许对 图像. 这些蛋白质聚合物的动力学模型可以 由不同的构象产生。 这种紊乱， 目前是结构确定的障碍， 用来揭示这些分子是如何随机波动的 并作为酶循环的一部分。 研究生物 结构应该是其中的主要受益者之一 技术.