NOVEL SCREEN FOR RESTRICTION ENDONUCLEASES

限制性核酸内切酶的新型筛选

• 批准号: 6180912
• 负责人: RICHARD J ROBERTS
• 金额: $ 35.54万
• 依托单位: NEW ENGLAND BIOLABS, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6180912
• 关键词: computer assisted sequence analysis; computer data analysis; computer program /software; computer system design /evaluation; data management; enzyme activity; molecular biology information system; nucleic acid sequence; restriction endonucleases

DESCRIPTION: (Adapted from the investigator's abstract) Restriction endonucleases are key bioreagents for genetic engineering. Although over 200 specificities exist currently, there is a need for additional enzymes that recognize novel sequences for mapping and other applications in molecular biology. The investigators propose to develop new, computationally-based approaches to screening public sequence databases to identify candidates for new restriction enzymes. The candidate genes identified by the computational methods will be screened biochemically to test predictions. New specificities will be fully characterized and offered for sale. PROPOSED COMMERCIAL APPLICATION: From this project we will discover new restriction enzymes that we will sale to the research community. If we are lucky, some of them may also be used in diagnostic application.

描述：（改编自研究者的摘要）限制性核酸内切酶是基因工程的关键生物试剂。尽管目前存在 200 多种特异性，但仍需要额外的酶来识别新序列，以用于分子生物学中的绘图和其他应用。研究人员建议开发新的、基于计算的方法来筛选公共序列数据库，以确定新限制性酶的候选者。通过计算方法识别的候选基因将通过生化筛选来测试预测。新的特性将被充分表征并出售。拟议的商业应用：通过这个项目，我们将发现新的限制性酶，并将其出售给研究界。如果幸运的话，其中一些也可以用于诊断应用。