NOVEL SCREEN FOR RESTRICTION ENDONUCLEASES

描述：（改编自研究者摘要）限制性内切酶是基因工程的关键生物试剂。虽然目前存在超过200种特异性，但仍需要识别新序列的其他酶，以用于分子生物学中的作图和其他应用。研究人员建议开发新的，基于计算的方法来筛选公共序列数据库，以确定新的限制性内切酶的候选者。通过计算方法确定的候选基因将进行生物化学筛选以测试预测。新的特性将被充分描述并提供销售。建议的商业应用：从这个项目中，我们将发现新的限制酶，我们将销售给研究界。如果我们幸运的话，其中一些也可以用于诊断应用。

DESCRIPTION: (Adapted from the investigator's abstract) Restriction endonucleases are key bioreagents for genetic engineering. Although over 200 specificities exist currently, there is a need for additional enzymes that recognize novel sequences for mapping and other applications in molecular biology. The investigators propose to develop new, computationally-based approaches to screening public sequence databases to identify candidates for new restriction enzymes. The candidate genes identified by the computational methods will be screened biochemically to test predictions. New specificities will be fully characterized and offered for sale. PROPOSED COMMERCIAL APPLICATION: From this project we will discover new restriction enzymes that we will sale to the research community. If we are lucky, some of them may also be used in diagnostic application.