U.S.-Australia Cooperative Research: Mechanisms of Evolutionary Change in Development

美澳合作研究：发展演变的进化机制

• 批准号: 8714075
• 负责人: Rudolf Raff
• 金额: $ 1万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8714075&HistoricalAwards=false
• 关键词: U.S.; Australia; Cooperative; Research; Mechanisms

This award will enable Professor Rudolph A. Raff from Indiana University to collaborate with Professor Donald T. Anderson at the University of Sydney, Australia. During two six-week visits by Dr. Raff to Sydney over a two-year period, various aspects of the early life history of two congeneric species of sea urchins having different pathways of development (with and without larval stage) will be examined. Studies will entail: (1) establishment of cell lineages by injection of fluorescent cell markers; (2) manipulations of isolated blastomeres in order to investigate cell committment and to determine the effects of nuclear/cytoplasmic ratio on cell cleavage dynamics; (3) preparation of cDNA libraries and monoclonal antibody probes; (4) examination of developmental variation in morphologically distinct populations of single species; and (5) comparative embryological descriptions of several species of directly developing sea urchins. The purpose of this research is to investigate the mechanisms for evolutionary change in early developmental processes using two congeneric species of sea urchins having different pathways of development as a model system. The research will benefit from the rather different, but complementary backgrounds of the U.S. and Australian investigators. Dr. Raff provides expertise on molecular and developmental aspects, while Dr. Anderson's background is in invertebrate evolution and development. The study organisms are unique to Australia and the resources at the University of Sydney allow for easy collection and culturing of the sea urchins.

该奖项将使印第安纳大学的鲁道夫·A·拉夫教授能够与澳大利亚悉尼大学的唐纳德·T·安德森教授合作。Raff博士在两年内对悉尼进行了两次为期六周的访问，期间将研究两种相同物种的海胆的早期生活史的不同方面，这些海胆具有不同的发育路径(有和没有幼虫阶段)。研究将包括：(1)通过注射荧光细胞标记建立细胞谱系；(2)操纵分离的卵裂球，以调查细胞承诺并确定核质比对细胞分裂动力学的影响；(3)制备cDNA文库和单抗探针；(4)检查单个物种在形态上不同的种群中的发育变异；以及(5)几种直接发育的海胆的比较胚胎学描述。本研究的目的是以两种发育路径不同的同类海胆为模型系统，研究早期发育过程中进化变化的机制。这项研究将受益于美国和澳大利亚调查人员截然不同但互补的背景。拉夫博士提供分子和发育方面的专业知识，而安德森博士的背景是无脊椎动物进化和发育。这些研究生物是澳大利亚独有的，悉尼大学的资源使海胆的收集和养殖变得容易。