Centers for Prokaryotic Genome Analysis and Molecular Phylogeny

该提案要求资金支持两个不同的，但 高度互动的生物设施中心： 基因组分析中心（CPGA） 分子系统发生学（CMP）。 正在创建CPGA以促进收购 和分析的DNA序列确定的基因组的 选择的古细菌以及真细菌 光合生物 该中心将是跨学科的， 它的科学家来自分子生物学、微生物 生理学、计算机科学、信息理论， 工程. 该中心的活动将产生重大影响， 对原核生物进化，分子生物学， 和生理学。 该中心还将大力参与 开发基于计算机的序列处理系统 数据 CMP是一个跨学科小组，其成员分享 对进化生物学感兴趣，并使用生物化学和 分子生物学方法来检测 各种各样的有机体。 除了核酸援助， 测序，核心成员具有国家认可的专业知识， 其他生物化学方法，特别是电泳， 免疫学 这两个团体有两个共同感兴趣的领域。演变和 序列分析的机制和方法。 的焦点 这些设施将用于基因组绘图、基因测序， 确定遗传学，并检查序列 核糖体RNA基因的进化。 仪器要求 将使伊利诺伊大学成为一个中心， 在分子进化方面的卓越成就

This proposal requests funds to support two distinct, yet highly interacting, biological facility centers: the Center for Prokaryotic Genome Analysis (CPGA) and the Center for Molecular Phylogeny (CMP). The CPGA is being created to facilitate the acquisition and analysis of the DNA sequences determined for the genomes of selected archaebacteria as well as an eubacterial photosynthetic organism. The Center will be interdisciplinary, its scientists being drawn from molecular biology, microbial physiology, computer science, information theory, and engineering. The Center's activities will have a major impact on the understanding of prokaryotic evolution, molecular biology, and physiology. The Center will also be heavily involved in developing computer.based systems for the handling of sequence data. The CMP is an interdisciplinary group whose members share interests in evolutionary biology and use biochemical and molecular biological methods to examine the phylogenies of diverse sets of organisms. In addition to nucleic aid sequencing, core members have nationally recognized epertise in other biochemical methods, especially electrophoresis and immunology. The two groups have two areas of common interest..evolution and the mechanics and methodology of sequence analysis. The focus of these facilities will be on genome mapping, gene sequencing, and the determination of phylogenies, and examination of the sequence evolution of ribosomal RNA genes. The instrumentation requested will enable the University of Illinois to become a center for excellence in molecular evolution.