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U.S.-France Cooperative Research: Biochemical and Genetic Dissection of Tn10 Transposon Termini

美法合作研究：Tn10 转座子末端的生化和遗传解剖

基本信息

批准号：
8715027
负责人：
Nancy Kleckner
金额：
$ 1.38万
依托单位：
Harvard University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1988
资助国家：
美国
起止时间：
1988-02-15 至 1993-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=8715027&HistoricalAwards=false
关键词：
U.S.France Cooperative Research Biochemical

项目摘要

This award will support collaborative research between Prof. Nancy Kleckner of Harvard University and Dr. Olivier Huisman of the Institut Pasteur, Paris, in the area of genetic analysis of mutant proteins. The objective of this work is the structural and functional dissection of DNA sequences at the ends of a gene segment, which is capable of moving from place to place in the chromosomes of an organism. Such a gene segment is called a transposon; the particular transposon to be studied in this work is called Tn10. Transposition of transposons leads to mutation, and the mutations which occur are to be studied by both genetic and biochemical methods. In the latter, assays for the binding of important proteins to specific DNA sequences should reveal the effects of specific mutations on known processes, and could show new intermediates in the reaction which might accumulate due to intermediate mutational steps. The French collaborators have considerable experience in genetic analysis. Additional materials, as well as expertise in use of an in vitro biochemical system for analysis of the Tn10 transposition, will be brought to the project by the U.S. investigator. The knowledge of complex interactions between DNA and proteins that is expected to result from this research could lead to enhanced progress in our understanding of the processes of the generation, and therefore of alteration, of life itself.

该奖项将支持合作研究，哈佛大学的Nancy Kleckner教授和巴黎巴斯德研究所的Olivier Huisman博士，突变蛋白的遗传分析领域。的这项工作的目标是结构和 DNA序列末端的功能解剖一个基因片段，它能够从一个地方植入生物体的染色体中。这样的基因片段称为转座子;特定的在这项工作中要研究的转座子被称为Tn 10。转座子的转座导致突变，发生的突变将由两个人研究，遗传学和生物化学方法。在后者中，用于重要蛋白质结合的测定特定的DNA序列应该揭示已知过程的特定突变，并且可以显示反应中的新中间体，由于中间突变步骤而积累。法国合作者有相当丰富的经验基因分析。其他材料，以及使用体外生化系统的专业知识， Tn 10转座的分析，将被带到美国调查员的项目。知识 DNA和蛋白质之间复杂的相互作用，预计这项研究的结果可能会导致在我们对《公约》的理解方面取得了更大的进展，生成的过程，因此，改变生活本身。