The Mechanism and Regulation of Ovulation in Fish

过去的研究涉及类花生酸，磷脂酰肌醇/蛋白质 鱼类排卵中激酶C系统和蛋白水解酶。该提案 研究将增加我们对这三个角色的理解， 鱼类排卵的介质。 具体而言，生产 鳟鱼和金鱼卵巢的脂氧代谢物将 研究使用放射性标记的前体掺入，然后 在高效液相色谱上分离代谢物。 的 类固醇诱导排卵期间初级前列腺素的产生 将使用放射免疫测定法对黄鲈的体内毒素进行研究。 一个 掺入系统，涉及标记滤泡 磷脂酰肌醇的放射性肌醇，将进行调查 以便将来的研究可以使用该系统来研究 卵泡肌醇磷酸激动剂刺激后。 总 卵泡蛋白水解活性在整个成熟和排卵过程中， 使用底物聚丙烯酰胺在溪鳟鱼和金鱼中进行跟踪 凝胶电泳 胶原蛋白溶解活性将随访至 使用特异性底物测定法在溪鳟卵泡中排卵， 确定这种蛋白酶在释放鸡蛋中的作用， 毛囊 目的是更好地了解内分泌 调节鱼类排卵的过程。

Past research implicates eicosanoids, the phosphatidylinositol/protein kinase C system and proteolytic enzymes in fish ovulation. The proposal research will increase our understanding of the role of these three mediators in fish ovulation. Specifically, the production of lipoxygense metabolites by brook trout and goldfish ovaries will be investigated using radio labeled precursor incorporation, followed by metabolite separation on high performance liquid chromatography. The production of primary prostaglandins during steroid-induced ovulation in yellow perch will be investigated using radioimmunoassay. An incorporation system, involving the labeling of follicular phosphatidylinositol by radioactive myoinositol, will be investigated so that future research can use this system to study the cycling of follicular inositol phosphates following agonist stimulation. Total follicular proteolytic activity thoughout maturation and ovulation will be followed in brook trout and goldfish using substrate polyacrylamide gel electrophoresis. Collagenolytic activity will be followed through ovulation in brook trout follicles using a specific substrate assay to determine the role of this proteases in the release of the eggs from the follicles. The goal is a better understanding of the endocrine processes regulating ovulation in fish.