Isolation and Characterization of Progestin Specific Messenger RNAs from the Yellow Perch Ovary at Ovulation

黄鲈排卵时卵巢中孕激素特异性信使 RNA 的分离和表征

• 批准号: 9408012
• 负责人: Frederick Goetz
• 金额: $ 16万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-15 至 1997-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408012&HistoricalAwards=false
• 关键词: Isolation; Characterization; Progestin; Specific; Messenger

9408012 Goetz Ovulation is the release of a mature egg from its enclosure in the ovary. Progestational steroids have been implicated in the control of ovulation but little is known about their specific role in this process. The objective of this proposal is to determine what these steroids are specifically stimulating in the ovary at the time of ovulation. To accomplish this, a fish model, the yellow perch, will be used. The reason for using this model is that research on perch has already demonstrated that ovulation is regulated by a progestational steroid and that the regulation requires the activation of genes and the synthesis of new protein. When a gene is activated in a cell, a specific message (called messenger RNA; mRNA) is created that acts as a template for protein synthesis. In the present study, the mRNAs that are stimulated by the progestational steroid during ovulation will be isolated using molecular biological techniques and their biochemical sequences determined. These sequences will be compared to those of known proteins that are maintained in the national GenBank database. Through these comparisons an initial idea of what the steroid is specifically stimulating at the time of ovulation can be obtained. ***

9408012戈茨排卵是将成熟的卵子从卵巢中的围栏中释放出来。孕激素与排卵的控制有关，但对其在这一过程中的具体作用知之甚少。这项建议的目的是确定这些类固醇在排卵时对卵巢有什么特别的刺激作用。为了实现这一点，将使用一种鱼模型--黄色鲈鱼。使用这个模型的原因是，对Perch的研究已经证明，排卵受到孕激素的调节，这种调节需要激活基因和合成新的蛋白质。当一个基因在细胞中被激活时，就会产生一种特定的信息(称为信使RNA；mRNA)，作为蛋白质合成的模板。在本研究中，将使用分子生物学技术分离排卵期间孕激素刺激的mRNAs，并测定它们的生化序列。这些序列将与国家基因库数据库中维护的已知蛋白质的序列进行比较。通过这些比较，可以初步了解类固醇在排卵时的具体刺激作用。***