Isolation and Characterization of Progestin Specific Messenger RNAs from the Yellow Perch Ovary at Ovulation

[408012 . Goetz]排卵是成熟的卵子从卵巢的外壳中释放出来。孕激素与控制排卵有关，但对其在这一过程中的具体作用知之甚少。这项建议的目的是确定这些类固醇在排卵时对卵巢的特异性刺激。为了做到这一点，将使用一种鱼类模型——黄鲈。使用该模型的原因是，对鲈鱼的研究已经证明，排卵是由孕激素调节的，并且这种调节需要激活基因和合成新的蛋白质。当一个基因在细胞中被激活时，一个特定的信息（称为信使RNA； mRNA）被创造出来，作为蛋白质合成的模板。在本研究中，我们将利用分子生物学技术对排卵期间孕激素刺激的mrna进行分离，并确定其生化序列。这些序列将与保存在国家基因库数据库中的已知蛋白质序列进行比较。通过这些比较，可以初步了解类固醇在排卵时的特异性刺激。＊＊＊

9408012 Goetz Ovulation is the release of a mature egg from its enclosure in the ovary. Progestational steroids have been implicated in the control of ovulation but little is known about their specific role in this process. The objective of this proposal is to determine what these steroids are specifically stimulating in the ovary at the time of ovulation. To accomplish this, a fish model, the yellow perch, will be used. The reason for using this model is that research on perch has already demonstrated that ovulation is regulated by a progestational steroid and that the regulation requires the activation of genes and the synthesis of new protein. When a gene is activated in a cell, a specific message (called messenger RNA; mRNA) is created that acts as a template for protein synthesis. In the present study, the mRNAs that are stimulated by the progestational steroid during ovulation will be isolated using molecular biological techniques and their biochemical sequences determined. These sequences will be compared to those of known proteins that are maintained in the national GenBank database. Through these comparisons an initial idea of what the steroid is specifically stimulating at the time of ovulation can be obtained. ***