Isolation and Characterization of Progestin Specific Messenger RNAs from the Yellow Perch Ovary at Ovulation

9408012戈茨 排卵是指成熟的卵子从卵巢中释放出来。 孕激素与控制排卵有关，但对其在此过程中的具体作用知之甚少。 这项建议的目的是确定这些类固醇在排卵时对卵巢有什么特异性刺激作用。 要做到这一点，鱼模型，黄色鲈鱼，将被使用。 之所以使用这种模型，是因为对鲈鱼的研究已经证明，排卵是由一种孕激素调节的，这种调节需要基因的激活和新蛋白质的合成。 当一个基因在细胞中被激活时，就会产生一种特定的信息（称为信使RNA; mRNA），作为蛋白质合成的模板。 在本研究中，促孕激素刺激排卵过程中的mRNA将使用分子生物学技术和它们的生化序列测定分离。 这些序列将与国家基因库数据库中保存的已知蛋白质序列进行比较。 通过这些比较，可以获得类固醇在排卵时具体刺激的初步想法。 ***

9408012 Goetz Ovulation is the release of a mature egg from its enclosure in the ovary. Progestational steroids have been implicated in the control of ovulation but little is known about their specific role in this process. The objective of this proposal is to determine what these steroids are specifically stimulating in the ovary at the time of ovulation. To accomplish this, a fish model, the yellow perch, will be used. The reason for using this model is that research on perch has already demonstrated that ovulation is regulated by a progestational steroid and that the regulation requires the activation of genes and the synthesis of new protein. When a gene is activated in a cell, a specific message (called messenger RNA; mRNA) is created that acts as a template for protein synthesis. In the present study, the mRNAs that are stimulated by the progestational steroid during ovulation will be isolated using molecular biological techniques and their biochemical sequences determined. These sequences will be compared to those of known proteins that are maintained in the national GenBank database. Through these comparisons an initial idea of what the steroid is specifically stimulating at the time of ovulation can be obtained. ***