Control of Genome Structure

基因组结构的控制

• 批准号: 8718163
• 负责人: Eric Selker
• 金额: $ 31万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8718163&HistoricalAwards=false
• 关键词: Control; Genome; Structure

The purpose of the proposed research is to characterize a novel genetic process in Neurospora crassa referred to by the acronym as "RIPing" (rearrangement induced premeiotically). Our preliminary work suggests that RIPing is responsible for rearranging certain duplicated DNA sequences at high frequency. The process also typically leads to methylation of the affected sequences, de novo. We will: (1) test the hypothesis that RIPing is triggered by sequence duplications; (2) determine the nature of the induced alterations; (3) explore why some sequences appear resistant to the process; (4) attempt to isolate mutants defective in RIPing: and (5) investigate whether RIPing is responsible for a class of spontaneous mutations. Characterization of this process may illuminate unrecognized genetic mechanisms, and at the same time should provide information of practical use to scientists making modifications to the genome. RIPing is almost certainly responsible for instability of transforming DNA in N. crassa, and it seems possible that a process analogous to RIPing is responsible for DNA rearrangements observed in other systems, such as in transgenic mice. It is likely that an understanding of RIPing will also give us insights into why the genome is organized the way it is.

拟议研究的目的是描述一种 粗糙脉孢菌中的新遗传过程， 首字母缩写为“RIPing”（减数分裂前诱导的重排）。 我们的初步工作表明，RIPing是负责 重组某些重复的DNA序列 频率. 这个过程通常也会导致甲基化 所有受影响的序列重新开始 我们将：（1）测试 假设RIPing是由序列重复触发的; (2)确定诱导的改变的性质;（3） 探索为什么有些序列对这个过程有抵抗力; (4)尝试分离RIPing缺陷突变体：和（5） 调查RIPing是否负责一类 自发突变 对这一过程的描述可能会揭示出 遗传机制，同时应提供 对科学家有实际用途的信息， 对基因组的修改。 几乎可以肯定， 在N.克拉萨， 似乎有可能是一个类似于RIPing的过程 负责在其他系统中观察到的DNA重排， 例如在转基因小鼠中。 很可能是一个 对RIPing的理解也会让我们深入了解为什么 基因组是这样组织的。