Control of Genome Structure

基因组结构的控制

• 批准号: 9019036
• 负责人: Eric Selker
• 金额: $ 33.9万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1995-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9019036&HistoricalAwards=false
• 关键词: Control; Genome; Structure

The goal of the project is to elucidate mechanisms of genetic instability in Neurospora. Knowledge gained from the project should shed light on several general areas: recombination, mutation, and evolution. In addition, the project has practical significance for Neurospora geneticists and researchers working on related fungi (e.g. plant pathogens). During the grant period we will: 1) determine whether RIP is responsible for low- frequency inactivation of duplicate genes in vegetative tissue; 2) attempt to derepress RIP, by mutations, in vegetative cells for use in subsequent genetic and biochemical experiments; 3) isolate mutants deficient in RIP; 4) determine if an inverted linked duplication is subject to RIP, premeiotic reciprocal recombination, or both (to provide a test of the idea that high- frequency premeiotic deletion of sequences between linked duplications results from reciprocal recombination and to provide information for a potential approach to isolate RIP mutants); 5) determine if unlinked duplications are subject to recombination processes ("gene conversion" and reciprocal exchange) prior to meiosis; 6) identify the postulated DNA-cytosine deaminase involved in RIP; 7) investigate the resistance to RIP of rDNA, and the effect of RIP on the transposon Tad. Filamentous fungi have an unusual process, known as RIP, by which they inactive all but one copy of most genes present in more than one copy. This process was discovered by the Principal Investigator during a previous NSF award. The mechanism and significance of this process are unclear, and the PI hopes to learn more about them during this award. A better understanding of RIP will lead to more effective methods for genetic manipulation of industrially important fungi.

该项目的目标是阐明脉孢菌遗传不稳定的机制。 从该项目中获得的知识应该能够阐明几个一般领域：重组、突变和进化。 此外，该项目对于脉孢菌遗传学家和研究相关真菌（例如植物病原体）的研究人员具有实际意义。 在资助期间，我们将：1）确定RIP是否导致营养组织中重复基因的低频失活； 2) 尝试通过突变在营养细胞中去抑制RIP，以用于后续的遗传和生化实验； 3) 分离RIP缺陷突变体； 4)确定反向连锁重复是否受到RIP、减数分裂前相互重组或两者的影响（以测试连锁重复之间序列的高频前减数分裂删除是由相互重组引起的，并为分离RIP突变体的潜在方法提供信息）； 5) 确定未连锁的重复在减数分裂之前是否经历重组过程（“基因转换”和相互交换）； 6) 鉴定假定的与 RIP 相关的 DNA-胞嘧啶脱氨酶； 7)研究rDNA对RIP的抗性，以及RIP对转座子Tad的影响。 丝状真菌有一个不寻常的过程，称为 RIP，通过该过程，它们使存在于多个拷贝中的大多数基因除了一个拷贝之外的所有基因都失活。 这个过程是首席研究员在之前的 NSF 奖项中发现的。 这个过程的机制和意义尚不清楚，PI希望在这次颁奖期间了解更多。 更好地了解 RIP 将带来更有效的方法来对工业上重要的真菌进行基因操作。