Probing the Structure of Ribosomal RNA

探索核糖体 RNA 的结构

• 批准号: 8718759
• 负责人: Walter Hill
• 金额: $ 36.48万
• 依托单位: University of Montana
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-15 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8718759&HistoricalAwards=false
• 关键词: Probing; Structure; Ribosomal; RNA

Ribosomes are key macromolecular components involved in the synthesis of proteins. They are made up of proteins and ribosomal RNAs. The goal of this proposal is to map the relative positions of single-stranded regions of rRNA that occur on the ribosome, using short, complimentary oligomers as probes. Interactions will be analyzed by fluorescence anisotropy, energy transfer techniques and photoaffinity labeling. The distance of selected sites from various ribosomal proteins will be measured by neutron diffraction. These studies will provide valuable information on the detailed structure of the ribosome and ribosomal subunits, and thus enhance our understanding of the process of protein synthesis.

核糖体是参与蛋白质合成的关键大分子成分。它们由蛋白质和核糖体RNA组成。这项提议的目标是利用短的互补低聚物作为探针，绘制出现在核糖体上的rRNA单链区域的相对位置。相互作用将通过荧光各向异性、能量转移技术和光亲和标记来分析。用中子衍射法测量不同核糖体蛋白的选定位置之间的距离。这些研究将提供关于核糖体和核糖体亚基详细结构的有价值的信息，从而加深我们对蛋白质合成过程的理解。