Equipment for a Modern Undergraduate Cell Biology Laboratory

现代本科细胞生物学实验室设备

• 批准号: 8750089
• 负责人: Leo Pezzementi
• 金额: $ 2.2万
• 依托单位: Birmingham Southern College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1989-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8750089&HistoricalAwards=false
• 关键词: Equipment; Modern; Undergraduate; Cell; Biology

In this project, students will be introduced to some of the instruments, techniques, and concepts of modern Cellular and Molecular Biology through a series of experiments performed with primary cultures of skeletal muscle cells prepared from chick embryos. The new equipment provided through this project consists of a biological safety cabinet and controlled-atmosphere incubator for the preparation, maintenance, and incubation of cell cultures of skeletal muscle (a cell type that differentiates in vitro and lends itself to a variety of cell biology experiments), an ultra-low-temperature freezer for the storage of perishable materials needed for cell culture, a liquid scintillation counter, micro-computer and associated software for the quantification, analysis, and presentation of the results of radiobiological experiments designed to investigate the rates of synthesis, degradation, transport, and secretion of muscle proteins, and three gel electrophoresis units for the determination of the molecular weight and identity of muscle- specific proteins. These experiments will allow students to use a variety of techniques from modern Cellular and Molecular Biology to explore some of the fundamental properties of living cells.

在这个项目中，学生将通过对鸡胚胎制备的骨骼肌细胞进行原代培养的一系列实验，了解现代细胞和分子生物学的一些仪器、技术和概念。本项目提供的新设备包括：用于制备、维护和培养骨骼肌细胞（一种体外分化的细胞类型，适合各种细胞生物学实验）的生物安全柜和可控气氛培养箱，用于储存细胞培养所需易腐材料的超低温冷冻室，液体闪烁计数器、微型计算机和相关软件，用于定量、分析和展示旨在研究肌肉蛋白质合成、降解、运输和分泌速率的放射生物学实验结果；三个凝胶电泳单元用于测定肌肉特异性蛋白的分子量和特性。这些实验将允许学生使用现代细胞和分子生物学的各种技术来探索活细胞的一些基本特性。