Equipment for a Modern Undergraduate Cell Biology Laboratory

在这个项目中，学生将被介绍给一些仪器， 现代细胞和分子生物学的技术和概念 通过一系列的实验进行了原代培养的 从鸡胚制备的骨骼肌细胞。 新装备 通过该项目提供的生物安全柜 以及用于制备、维持 和骨骼肌细胞培养物（一种细胞类型， 在体外分化并适用于各种细胞生物学 实验），一个超低温冷冻机的存储， 细胞培养所需的易腐材料，液体闪烁 计数器、微型计算机和用于定量的相关软件， 放射生物学实验结果的分析和介绍 旨在研究合成，降解，运输， 和肌肉蛋白质的分泌，以及三个凝胶电泳单元 用于测定肌肉的分子量和特性- 特定蛋白质 这些实验将允许学生使用各种技术 从现代细胞和分子生物学中探索一些 活细胞的基本特性。

In this project, students will be introduced to some of the instruments, techniques, and concepts of modern Cellular and Molecular Biology through a series of experiments performed with primary cultures of skeletal muscle cells prepared from chick embryos. The new equipment provided through this project consists of a biological safety cabinet and controlled-atmosphere incubator for the preparation, maintenance, and incubation of cell cultures of skeletal muscle (a cell type that differentiates in vitro and lends itself to a variety of cell biology experiments), an ultra-low-temperature freezer for the storage of perishable materials needed for cell culture, a liquid scintillation counter, micro-computer and associated software for the quantification, analysis, and presentation of the results of radiobiological experiments designed to investigate the rates of synthesis, degradation, transport, and secretion of muscle proteins, and three gel electrophoresis units for the determination of the molecular weight and identity of muscle- specific proteins. These experiments will allow students to use a variety of techniques from modern Cellular and Molecular Biology to explore some of the fundamental properties of living cells.