A Collaborative Recombinant DNA Technology Course with Laboratory

与实验室合作的重组DNA技术课程

• 批准号: 9950225
• 负责人: Leo Pezzementi
• 金额: $ 2.04万
• 依托单位: Birmingham Southern College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9950225&HistoricalAwards=false
• 关键词: Collaborative; Recombinant; DNA; Technology; Course

Both the classroom and laboratory portions of this course in recombinant DNA technology are designed to promote contextual, collaborative, inquiry-based learning of science. Students teach one another and have a sense of ownership of their education. In class, emphasis is on group presentations and critical reading and discussion of scientific articles that use recombinant DNA technology to address questions in both basic and applied research. In the laboratory, investigative projects are designed to help students pose and answer two questions: 1) How do you clone a gene? and 2) And so then what do you do with it? The two projects are random DNA cloning, and in vitro expression and site-directed mutagenesis of cholinesterase; the latter project integrates the research and teaching of the principal investigator. Students present the results of their two laboratory projects in a poster and in a paper with the format of a scientific article. It adapts principals found in Project Kaleidoscope (1991, What works: building natural science communities Volume one) and the PEW Higher Education Collaborative (1998, Policy perspectives sponsored by the PEW science program in undergraduate education. University of Pennsylvania, Phila. PA), and the work of Brian Coppola (1997, J. Chem Ed. 74, 74-83 and 84-94) and the Council on Undergraduate Research (1995, CUR Quarterly 16, 109-114).

重组DNA技术课程的课堂和实验室部分旨在促进科学的上下文，协作，探究式学习。 学生们互相教导，对自己的教育有一种主人翁感。在课堂上，重点是小组报告和批判性的阅读和科学文章的讨论，使用重组DNA技术来解决基础和应用研究中的问题。在实验室里，研究项目旨在帮助学生提出并回答两个问题：1）如何克隆基因？2）然后你用它做什么？这两个项目分别是随机DNA克隆和胆碱酯酶的体外表达和定点突变;后者项目整合了主要研究者的研究和教学。学生们以海报和科学文章的格式在论文中展示他们两个实验项目的结果。它适应在万花筒项目（1991年，什么工作：建立自然科学社区第一卷）和PEW高等教育合作（1998年，政策观点由PEW科学计划在本科教育赞助的校长。 宾夕法尼亚大学，费城。PA），以及Brian Coppola（1997，J. Chem Ed.74，74-83和84-94）和本科生研究理事会（1995，CUR Quarterly 16，109-114）的工作。