Equipment to Expand Undergraduate Instruction in Cellular and Molecular Biology.

扩大细胞和分子生物学本科教学的设备。

• 批准号: 8750217
• 负责人: William Harvey
• 金额: $ 3.45万
• 依托单位: Earlham College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-15 至 1989-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8750217&HistoricalAwards=false
• 关键词: Equipment; Expand; Undergraduate; Instruction; Cellular

The Biology Department is involved in a four-phase program to expand the instrumentation of its Cellular and Molecular Biology laboratories. Phase one was completed by the institution; this project is covering the second phase which involves procurement of an ultracentrifuge, electrophoresis equipment, photographic equipment, a uv/visible spectrophotometer, a CO2 incubator, and an ion-free reagent water system. This equipment enables undergraduates to perform expanded laboratory experiments and individual projects in cell fractionation; protein, DNA and RNA separations; transfers of proteins and nucleic acids for testing and analysis (Southern, Northern, and Western blots); quantitative determination of proteins and nucleic acids; autoradiography; DNA sequencing; restriction digests of nucleic acids; and cloning of restriction fragments. A new course in Molecular Genetics is being offered, while major laboratory segments of Advanced Cell Biology and Developmental Biology are being expanded. Subtantial laboratory exercises also are being added to coursework in Microbiology, Genetics, Biochemistry, and Immunology. Students are gaining vastly expanded experience with current techniques and equipment used in Protein and Nucleic Acid Biochemistry and Molecular Genetics.

生物系参与了一个分四个阶段的计划，以扩大其细胞和分子生物学实验室的仪器。第一阶段由该机构完成；该项目包括第二阶段，涉及采购超离心机、电泳仪、摄影设备、紫外线/可见光分光光度计、二氧化碳培养箱和无离子试剂水系统。该设备使本科生能够在细胞分离；蛋白质、DNA和RNA分离；用于测试和分析的蛋白质和核酸的转移(Southern、Northern和Western blotts)；蛋白质和核酸的定量测定；放射自显影；DNA测序；核酸限制性内切酶；以及限制片段的克隆。正在开设一门新的分子遗传学课程，同时高级细胞生物学和发育生物学的主要实验室部分正在扩大。微生物学、遗传学、生物化学和免疫学的课程中也增加了精妙的实验室练习。学生们在蛋白质和核酸生物化学和分子遗传学的现有技术和设备方面获得了广泛的经验。