Gene Targeting Via Site Specific Recombination in Plant Cells

该项目拟对植物体细胞基因靶向的可能性进行评估。该实验首先引入一个突变选择标记基因，并建立一组稳定的转化子。然后将这些突变系与相同选择标记基因的互补突变进行二次转化。在这个时候，选择将被施加，以识别转化的细胞，在这些互补突变体之间存在位点特异性重组，从而重建一个功能可选择的标记基因。我们将研究这一事件的分子细节。该技术可以在植物细胞中进行基因替换或失活，并将顺序引入的性状靶向到单个位点，从而加快植物品种的改良。

The PI proposes to evaluate the possibility of gene targeting in somatic plant cells. The experiment involves first the introduction of a mutant selectable marker gene and the establishment of a set of stable transformants. These mutant lines will then be secondarily transformed with a complimentary mutant in the same selectable marker gene. At this time selection will be imposed so as to identify transformed cells in which there has been site specific recombination between these complimentary mutants thereby reconstructing a functional selectable marker gene. The molecular details of this event will be examined. This technology could allow for gene replacement or inactivation in plant cells and targeting of sequentially introduced traits to a single locus to expedite plant variety improvement.