Gene Targeting Via Site Specific Recombination in Plant Cells

通过植物细胞中的位点特异性重组进行基因靶向

• 批准号: 8760003
• 负责人: Pamela Dunsmuir
• 金额: $ 4.76万
• 依托单位: DNA Plant Technologies, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8760003&HistoricalAwards=false
• 关键词: Gene; Targeting; Via; Site; Specific

The PI proposes to evaluate the possibility of gene targeting in somatic plant cells. The experiment involves first the introduction of a mutant selectable marker gene and the establishment of a set of stable transformants. These mutant lines will then be secondarily transformed with a complimentary mutant in the same selectable marker gene. At this time selection will be imposed so as to identify transformed cells in which there has been site specific recombination between these complimentary mutants thereby reconstructing a functional selectable marker gene. The molecular details of this event will be examined. This technology could allow for gene replacement or inactivation in plant cells and targeting of sequentially introduced traits to a single locus to expedite plant variety improvement.

该项目负责人建议评估植物体细胞基因靶向的可能性。 该实验首先涉及引入突变选择标记基因并建立一组稳定的转化体。 然后，这些突变体系将用相同选择性标记基因中的互补突变体进行二次转化。 此时，将进行选择以鉴定转化细胞，其中这些互补突变体之间存在位点特异性重组，从而重建功能性选择标记基因。 该事件的分子细节将被检查。 该技术可以实现植物细胞中的基因替换或失活，并将连续引入的性状靶向单个基因座，以加快植物品种改良。