Genetic Engineering of Plants with Antifreeze Proteins to Enhance the Quality of Frozen Plant Products

具有抗冻蛋白的植物基因工程可提高冷冻植物产品的质量

• 批准号: 9105163
• 负责人: Pamela Dunsmuir
• 金额: $ 22.43万
• 依托单位: DNA Plant Technologies, Inc.
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-10-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105163&HistoricalAwards=false
• 关键词: Genetic; Engineering; Plants; Antifreeze; Proteins

The aim of the research described is to develop transformed plant varieties with enhanced freezing tolerance through the expression of antifreeze proteins that inhibit ice crystal growth. We have shown that tobacco and tomato plants transformed with a winter flounder antifreeze gene express high levels of the corresponding mRNA. Furthermore, the gene was modified so that the encoded protein is larger and more readily detected. In plants containing this fusion protein, antifreeze expression was observed at the RNA, protein and activity level, with transcriptional levels predominating. We will now evaluate the impact of antifreeze protein on the freezing tolerance properties of fruit from transgenic plants as well as from nontransgenic fruits that have been infiltrated with antifreeze protein. If necessary, we will optimize the protein concentration in plant tissue by enhancing protein activity, protein stability, transcription, and translatability. Constructions resulting in optimal freezing tolerance will be transferred into appropriate commercial varieties.***//

所述研究的目的是开发转化植物 通过表达具有增强的抗冻性的品种 抑制冰晶生长的抗冻蛋白质。 我们有 表明烟草和番茄植物转化了冬季 比目鱼抗冻基因表达高水平的相应 mRNA。 此外，基因被修改，使编码的 蛋白质更大，更容易检测。 在含有 在该融合蛋白的RNA上观察到抗冻剂表达， 蛋白质和活性水平，转录水平 占主导地位。 我们现在将评估抗冻蛋白对 转基因植物果实的抗冻性 以及从非转基因水果中， 抗冻蛋白 如有必要，我们将优化蛋白质 通过增强蛋白质活性在植物组织中浓缩， 蛋白质稳定性、转录和可翻译性。 导致最佳抗冻性的结构将是 转成适当的商业品种。*//