Genetic Engineering of Plants for Bacterial Disease Resistance

植物抗细菌病害基因工程

• 批准号: 8860473
• 负责人: Pamela Dunsmuir
• 金额: $ 4.93万
• 依托单位: DNA Plant Technologies, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1989-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8860473&HistoricalAwards=false
• 关键词: Genetic; Engineering; Plants; Bacterial; Disease

The research described in this proposal will test the feasibility of expressing the antibacterial protein cecropin in plant cells. The gene will be synthesized, and then the peptide produced in E. coli. This protein will be used in antibody preparation and in lethality tests against bacterial plant pathogens. The cecropin gene will then be engineered for expression in plant cells, and introduced into tomato leaf disc cells by Agrobacterium transformation. Transformed callus tissue and transformed plants will be evaluated for the expression of cecropin protein. The research has the potential of enabling the production of vegetable crops with resistance to post-harvest disease.

本提案中所述的研究将测试以下方面的可行性： 在植物细胞中表达抗菌蛋白天蚕素。 基因 将被合成，然后在E.杆菌 这 蛋白质将用于抗体制备和致死性试验 对抗植物病原细菌。 然后天蚕素基因将 在植物细胞中表达，并导入番茄中， 通过农杆菌转化法转化叶盘细胞。 转化愈伤组织 组织和转化的植物将被评估用于表达 天蚕素蛋白 这项研究有可能使蔬菜生产 抗收获后病害的作物。