Expression and Targetting of Antifreeze Protiens in Transgenic Plants

转基因植物中抗冻蛋白的表达和靶向

• 批准号: 8961029
• 负责人: Pamela Dunsmuir
• 金额: $ 4.94万
• 依托单位: DNA Plant Technologies, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1990-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8961029&HistoricalAwards=false
• 关键词: Expression; Targetting; Antifreeze; Protiens; Transgenic

The quality of plant products which have been frozen for storage can be compromised by the damaging effects of ice growth within the frozen tissue. A number of proteins have been identified which can inhibit ice crystal growth when present in very low concentrations. We will study a gene for one of these proteins cloned into plants. In addition, the gene will be modified so that the protein it codes for is larger and more easily detected (by fusion to protein A from Staphylococcus aureus), and is secreted from plant cells (by the attachment of secretion signal sequences). The ability of the transformed plants to express and localise the fusion proteins will be determined. Simple assays which measure the damage caused by ice crystal growth in frozen plant tissue will be used to assess the ability of the protein to protect against this damage. This approach offers a novel way of improving the quality of frozen fruits and vegetables, and increasing the range of fruits and vegetables that can be successfully frozen.

冷冻储存的植物产品的质量可能会因冷冻组织内冰生长的破坏性影响而受到影响。 已鉴定出许多蛋白质在浓度非常低时可以抑制冰晶生长。 我们将研究克隆到植物中的其中一种蛋白质的基因。 此外，该基因将被修改，使其编码的蛋白质更大且更容易检测（通过与金黄色葡萄球菌的蛋白质A融合），并从植物细胞中分泌（通过附着分泌信号序列）。 将测定转化植物表达和定位融合蛋白的能力。 测量冷冻植物组织中冰晶生长造成的损害的简单测定将用于评估蛋白质防止这种损害的能力。 这种方法提供了一种提高冷冻水果和蔬菜质量并增加可成功冷冻的水果和蔬菜范围的新方法。