Cloning of Hybrid Celery Using Bioreactor Technology

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This proposal describes research on bioreactor scale up of plant cell culture directed toward plant propagation. In Phase I research, the PI proposes to clone a breeder's cross hybrid of celery in a 1 to 4 liter bioreactor. Using this system, he proposes to identify the importance of agitation, aeration, and time in liquid culture on the yield and performance of developing celery somatic embryos. He further proposes to determine the level of singulation and synchronization of embryos resulting from regeneration in the bioreactor. A method for separating embryos from debris and immature embryos will be determined. Finally, several hundred somatic embryos will be regenerated and transplanted to the field. During Phase II research, he proposes to complete the field evaluation of plant variation as well as continue bioreactor studies. Development of these techniques could enable commercialization and delivery of hybrid crops where hybrid seed production methods do not currently exist.