Solids NMR Characterization of Bacterial Cell Walls

细菌细胞壁的固体核磁共振表征

• 批准号: 8803687
• 负责人: G. Edwin Wilson
• 金额: $ 23.65万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803687&HistoricalAwards=false
• 关键词: Solids; NMR; Characterization; Bacterial; Cell

This project is designed to continue the development of solids state NMR as a tool for characterization of bacterial peptidoglycan, specifically the peptidoglycan of B. subtilis. Both whole cells and cell walls of bacteria grown in defined media containing 13C and 15N -labeled amino acids will be examined by solids state NMR for the identification and quantitative analysis of cell wall substructures. Gas chromatograph-mass spectroscopy techniques will be developed to complement the NMR data and will allow complete analyses in the presence of label scrambling. The bacterial cell wall is vital for the existence of these organisms in the environment and its metabolism represents a major focus for attack by antibiotics. Recent NMR experiments aimed at developing a new analytical technique for determination of cell-wall cross-linking were carried out on the bacterium A. viridans. This bacterium transports both labeled alanine and lysine readily and incorporates them into the cell wall with no scrambling. NMR can then be used to measure directly the cross-link density in the bacterial cell wall. An important step in the development of solids state NMR as a tool for the characterization of peptidoglycan substructures in bacterial cell walls is an extension of the technique to bacteria which are more commonly studied, e.g. B. Subtilis.***//

该项目旨在继续发展 固体核磁共振作为表征细菌的工具 肽聚糖，特别是B的肽聚糖。枯草杆菌。 细菌的整个细胞和细胞壁都生长在限定的 将含有13 C和15 N标记的氨基酸的培养基 通过固态NMR进行鉴定， 细胞壁亚结构的定量分析。 气体 将开发色谱-质谱技术 以补充核磁共振数据， 在标签加扰的存在下。 细菌细胞壁对于这些细菌的存在至关重要。 环境中的生物及其代谢代表了 抗生素攻击的主要焦点。 最近的NMR 旨在开发新分析技术的实验 测定细胞壁交联进行了 细菌A。草绿色 这种细菌将两者都 标记的丙氨酸和赖氨酸，并将它们掺入 没有干扰的细胞壁 NMR可以用来 直接测量细菌细胞中的交联密度 墙 固态发展的重要一步 NMR作为肽聚糖表征的工具 细菌细胞壁中的亚结构是 技术对细菌更常见的研究，例如， B。* //