Assessing and Manipulating Single-Cell Productivity in Recombinant Cell Populations

评估和操纵重组细胞群中的单细胞生产力

• 批准号: 8805636
• 负责人: James Bailey
• 金额: $ 38.98万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8805636&HistoricalAwards=false
• 关键词: Assessing; Manipulating; Single; Cell; Productivity

This research involves three parallel and conceptually related studies to explore the bases for heterogeneity and single-cell productivity in recombinant microbial populations. It also seeks to develop quantitative understandings of the relationships between genetic and environmental parameters and this heterogeneity. The research involves very recent developments in experimental methodology for rapid single-cell measurements and will contribute to further expansion and broader application of these extremely efficient and informative measurements. E. coli and yeast cells will also be studied using a set of experimental systems which have been developed recently in the biotechnology industry. Three graduate students will conduct the proposed experiments and will be in direct collaboration with a local biotechnology company.

这项研究包括三个平行的和概念上相关的研究，以探索重组微生物种群中异质性和单细胞生产率的基础。它还试图发展对遗传和环境参数与这种异质性之间的关系的量化理解。这项研究涉及用于快速单细胞测量的实验方法的最新发展，并将有助于这些极其有效和信息丰富的测量的进一步扩展和更广泛的应用。还将使用生物技术行业最近开发的一套实验系统来研究大肠杆菌和酵母细胞。三名研究生将进行拟议的实验，并将与当地一家生物技术公司直接合作。