Immobilized Cell Bioconversion Catalysis: Manipulation and Monitoring of Activity, Selectivity, and Stability

固定化细胞生物转化催化：活性、选择性和稳定性的操作和监测

• 批准号: 8604408
• 负责人: James Bailey
• 金额: $ 23.54万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-15 至 1989-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8604408&HistoricalAwards=false
• 关键词: Immobilized; Cell; Bioconversion; Catalysis; Manipulation

The objective of this research is to understand the response of whole-cell catalysts to immobilization and to subsequent operating environments. In this study, relationships between the immobilization method used and the catalyst properties which result will be established. Techniques to be used include fluorometry to monitor intracellular reduced pyridine nucleotide levels, NMR to investigate metabolism and bioenergetics, and flow cytometry to monitor population dynamics. Application of these techniques to immobilized cells is novel.

本研究的目的是了解 全细胞催化剂的固定化和随后的操作 环境. 在这项研究中， 使用的方法和催化剂的性质，其结果将是 确立了习 使用的技术包括荧光测定法， 细胞内还原吡啶核苷酸水平，核磁共振研究 代谢和生物能量学，以及流式细胞术来监测种群 动力学 这些技术在固定化细胞中的应用是 小说