Engineering Research Equipment Grant: Equipment for Construction and Characterization of Recombinant Microorganisms

工程研究设备补助金：重组微生物的构建和表征设备

• 批准号: 8607589
• 负责人: James Bailey
• 金额: $ 4.36万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-15 至 1987-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8607589&HistoricalAwards=false
• 关键词: Engineering; Research; Equipment; Grant; Construction

Equipment regarded as essential to the fundamental understanding of the interactions among recombinant DNA molecules, host cells, and bioreactor operating conditions is purchased. To conduct research on these interactions, it is important to possess the capability for constructing the recombinant DNA molecules of interest and for studying cells which harbor these recombinant DNA molecules of interest and for studying cells which harbor these recombinant DNA molecules (plasmids). This equipment will be empolyed in several research projects, including the genetic engineering of subtrate utilization capabilities, studies on the secretion of cloned-gene products from genetically altered cells, investigations of plasmid stability and copy number control, and research on the induction kinetics and efficiencies of regulated promoters. In each of these projects, plasmids not presently available need to be constructed.

购买的设备被认为是对重组DNA分子、宿主细胞和生物反应器操作条件之间相互作用的基本理解所必需的。为了进行这些相互作用的研究，重要的是要具备构建感兴趣的重组DNA分子和研究含有这些感兴趣的重组DNA分子的细胞以及研究含有这些重组DNA分子（质粒）的细胞的能力。该设备将用于几个研究项目，包括底物利用能力的基因工程，基因改造细胞克隆基因产物分泌的研究，质粒稳定性和拷贝数控制的研究，以及调控启动子的诱导动力学和效率的研究。在每一个项目中，都需要构建目前无法获得的质粒。