Engineering Research Equipment Grant: Equipment for Construction and Characterization of Recombinant Microorganisms

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Equipment regarded as essential to the fundamental understanding of the interactions among recombinant DNA molecules, host cells, and bioreactor operating conditions is purchased. To conduct research on these interactions, it is important to possess the capability for constructing the recombinant DNA molecules of interest and for studying cells which harbor these recombinant DNA molecules of interest and for studying cells which harbor these recombinant DNA molecules (plasmids). This equipment will be empolyed in several research projects, including the genetic engineering of subtrate utilization capabilities, studies on the secretion of cloned-gene products from genetically altered cells, investigations of plasmid stability and copy number control, and research on the induction kinetics and efficiencies of regulated promoters. In each of these projects, plasmids not presently available need to be constructed.