Synthesis of the Chlorophyll Isocyclic Ring

这个项目有4个具体目标。1. 完成叶绿素6-B- oh中间体的立体化学研究。2. 利用18O2培养箱研究混合功能加氧酶对原叶绿内酯的酶促反应。3. 对6- b -酮丙酸甲酯、7-丙酸进行代谢研究，测定可溶性叶绿体蛋白和叶绿体膜片段催化原叶绿内酯的形成。4. 确定单酯环化所需的酶的数量。发育中的叶绿体将被分离，以研究这些代谢转化与无细胞器（可能是可溶性的）提取物。本研究探讨了所有植物和细菌叶绿素的一个普遍特征的生物合成；一个环的形成。叶绿素是光合作用的重要催化剂

This project has 4 specific objectives. 1. To complete a study on the stereochemistry of the 6-B- OH-intermediate of chlorophyll. 2. To study the enzymatic formation of protochlorophyllide by a mixed function oxygenase using 18O2 incubators. 3. To conduct metabolic studies with 6-B-ketopropionate methyl ester, 7-propionic acid to assay for protochlorophyllide formation catalyzed by soluble chloroplast proteins and fragments of chloroplast membranes. 4. To determine the number of enzymes required for the cyclization using monoesters. The developing chloroplasts will be fractionated to study these metabolic transformations with organelle-free (and possibly soluble) extracts.%%% This research investigates the biosynthesis of a universal feature of all plant and bacterial chlorophylls; the formation of one of the rings. Chlorophylls are the essential catalysts in photosynthesis.***//