Regulation of Ornithine Transcarbamylase and Its Function inPlant Nitrogen Metabolism

几个主要的氮代谢途径受到影响 通过精氨酸生物合成途径的活动。 在 植物，很少有人知道关于代谢调节 精氨酸生物合成 同样，监管 鸟氨酸转氨甲酰酶（OTC）的活性，这种酶 催化精氨酸合成的第一步 生物合成途径未知。 氮的动态 分配给精氨酸、嘧啶核苷酸和 多胺生物合成途径将在 不同的生理条件下，使用放射性标记的 这些途径的前体和特异性抑制剂。 这些 实验将在两个模型系统（烟草）中进行 用含胭脂碱合酶的Ti质粒转化 pGV 3850和干旱胁迫的燕麦叶），其中高 精氨酸在组织中的利用率是预期的 支持精氨酸途径和OTC活性升高。% 植物的氮代谢受到影响时， 压力很大 本项目研究了多胺的合成途径 生物合成及其在胁迫期间如何调节。 这 会让我们更好地理解 在工厂的地方。 干预措施将有可能 保护植物的生产力。*//

Several of the major nitrogen metabolic pathways are impacted by activities of the arginine biosynthetic pathway. In plants, little is known regarding the metabolic regulation of arginine biosynthesis. Similarly, mechanisms regulating the activity of ornithine transcarbamylase (OTC), the enzyme that catalyzes the first committed step in the arginine biosynthetic pathway, are unknown. The dynamics of nitrogen allocation to the arginine, pyrimidine nucleotide and polyamine biosynthetic pathways will be investigated under different physiological conditions, using radiolabeled precursors and specific inhibitors of these pathways. These experiments will be carried out in two model systems (tobacco transformed with the nopaline synthase containing Ti plasmid pGV3850 and osmotically-stressed oat leaf) in which high rates of arginine utilization in the tissue would be expected to support elevated arginine pathway and OTC activities.%%% Nitrogen metabolism in plants is affected when the plant is stressed. This project investigates the pathway of polyamine biosynthesis and how it is regulated during stress. This will lead to a better understanding of the changes taking place in the plant. Interventions will be possible to protect plant productivity.***//