Regulation of Ornithine Transcarbamylase and Its Function inPlant Nitrogen Metabolism

鸟氨酸转氨甲酰酶的调控及其在植物氮代谢中的功能

• 批准号: 8806240
• 负责人: Robert Slocum
• 金额: $ 9.2万
• 依托单位: Williams College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1992-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8806240&HistoricalAwards=false
• 关键词: Regulation; Ornithine; Transcarbamylase; Its; Function

Several of the major nitrogen metabolic pathways are impacted by activities of the arginine biosynthetic pathway. In plants, little is known regarding the metabolic regulation of arginine biosynthesis. Similarly, mechanisms regulating the activity of ornithine transcarbamylase (OTC), the enzyme that catalyzes the first committed step in the arginine biosynthetic pathway, are unknown. The dynamics of nitrogen allocation to the arginine, pyrimidine nucleotide and polyamine biosynthetic pathways will be investigated under different physiological conditions, using radiolabeled precursors and specific inhibitors of these pathways. These experiments will be carried out in two model systems (tobacco transformed with the nopaline synthase containing Ti plasmid pGV3850 and osmotically-stressed oat leaf) in which high rates of arginine utilization in the tissue would be expected to support elevated arginine pathway and OTC activities.%%% Nitrogen metabolism in plants is affected when the plant is stressed. This project investigates the pathway of polyamine biosynthesis and how it is regulated during stress. This will lead to a better understanding of the changes taking place in the plant. Interventions will be possible to protect plant productivity.***//

几种主要的氮代谢途径受到精氨酸生物合成途径活性的影响。 在植物中，人们对精氨酸生物合成的代谢调节知之甚少。 同样，鸟氨酸转氨甲酰酶 (OTC) 的活性调节机制尚不清楚，鸟氨酸转氨甲酰酶是催化精氨酸生物合成途径中第一个关键步骤的酶。 将使用放射性标记的前体和这些途径的特异性抑制剂，在不同的生理条件下研究精氨酸、嘧啶核苷酸和多胺生物合成途径的氮分配动态。 这些实验将在两个模型系统（用含有 Ti 质粒 pGV3850 的胭脂碱合酶转化的烟草和渗透胁迫的燕麦叶）中进行，其中组织中精氨酸的高利用率预计将支持精氨酸途径和 OTC 活性的升高。%%% 当植物受到胁迫时，植物中的氮代谢受到影响。 该项目研究多胺生物合成途径及其在应激过程中的调节方式。 这将有助于更好地了解工厂中发生的变化。 干预措施将有可能保护工厂生产力。***//