RUI: Regulation of Putrescine and Proline Metabolism in Response to Plant Stress

RUI：植物胁迫下腐胺和脯氨酸代谢的调节

• 批准号: 9117814
• 负责人: Robert Slocum
• 金额: $ 14.38万
• 依托单位: Goucher College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-15 至 1994-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9117814&HistoricalAwards=false
• 关键词: RUI; Regulation; Putrescine; Proline; Metabolism

The goal of the proposed project is to understand the metabolic controls on proline and putrescine synthesis in two plants (pea, oat) that exhibit fundamentally different responses to osmotic stress. Oat leaves accumulate putrescine to high levels, while proline accumulation is minimal. Pea leaves accumulate proline, but very little putrescine. Radiolabeled precursors and inhibitors of the arginine, putrescine and proline biosynthetic pathways will be used to investigate basic metabolic responses to stress in these plants. Cellular and molecular mechanisms regulating the activities of the arginine decarboxylase (ADC), which catalyzes the first reaction in the putrescine biosynthetic pathway, and pyrroline-5-carboxylate reductase (P5CR), an enzyme which carries out the final step in proline biosynthesis will be investigated. There is evidence that the activities of both ADC and P5CR are stress-inducible and these enzymes may play important roles in the regulation of proline and putrescine accumulation in response to stress. A more complete understanding of stress-dependent changes in these pathways may yield new insights into basic underlying stress adaptation strategies used by plants and may lead to the development of cultivars with improved stress tolerance characteristics.

该项目的目标是了解两种植物（豌豆、燕麦）对脯氨酸和腐胺合成的代谢控制，这两种植物对渗透胁迫表现出根本不同的反应。 燕麦叶积累大量腐胺，而脯氨酸积累很少。 豌豆叶子积累脯氨酸，但腐胺含量很少。 精氨酸、腐胺和脯氨酸生物合成途径的放射性标记前体和抑制剂将用于研究这些植物对胁迫的基本代谢反应。 将研究调节精氨酸脱羧酶（ADC）（催化腐胺生物合成途径中的第一个反应）和吡咯啉-5-羧酸还原酶（P5CR）（一种进行脯氨酸生物合成最后步骤的酶）活性的细胞和分子机制。 有证据表明 ADC 和 P5CR 的活性都是应激诱导的，并且这些酶可能在响应应激而调节脯氨酸和腐胺积累中发挥重要作用。 对这些途径中胁迫依赖性变化的更全面的了解可能会对植物使用的基本胁迫适应策略产生新的见解，并可能导致具有改善的胁迫耐受特性的品种的开发。