Research Initiation: Investigation of the Mechanisms Responsible for Increased Antibody Production by Hybridomas Exposed to Stress

研究启动：研究应激环境下杂交瘤抗体产量增加的机制

• 批准号: 8809244
• 负责人: William Miller
• 金额: $ 7.86万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8809244&HistoricalAwards=false
• 关键词: Research; Initiation; Investigation; Mechanisms; Responsible

RESEARCH INITIATION AWARD: INVESTIGATION OF THE MECHANISMS RESPONSIBLE FOR INCREASED ANTIBODY PRODUCTION BY HYBRIDOMAS EXPOSED TO STRESS ABSTRACT Monoclonal antibodies are among the largest-volume products made using mammalian cells. Increased antibody production has been reported for hybridomas exposed to environmental stress. The objectives of this project are to characterize the mechanisms responsible for increased antibody production, and to identify reactor conditions with increased productivity. Concurrent changes in energy metabolism and DNA synthesis will be monitored in order to determine the interrelationships between the regulation of cell growth, energy production and protein expression. An understanding of these relationships is required in order to decouple cell growth from product formation. Excessive cell growth is not desired because expensive medium is wasted on the production of biomass. Reduced cell growth rates are also desirable because many primary, hybrid, and genetically- altered cell lines decrease their rate of product formation after repeated cell divisions.

研究启动奖： 调查造成缔约方会议工作人员 通过暴露于应激的杂交瘤产生抗体 摘要 单克隆抗体是产量最大的产品之一 使用哺乳动物细胞。 增加的抗体产量 报道了暴露于环境压力的杂交瘤。 的 本项目的目标是描述这些机制的特点， 负责增加抗体产生，并确定 反应器条件，提高生产率。 能量代谢和DNA合成的同时变化将是 监测，以确定之间的相互关系， 调节细胞生长、能量产生和蛋白质 表情 需要了解这些关系 以使细胞生长与产物形成分离。 过度的细胞生长是不期望的，因为昂贵的培养基是不期望的。 浪费在生物质的生产上。 细胞生长速率降低 也是可取的，因为许多主要的，杂交，和遗传- 改变的细胞系降低了它们的产物形成速率， 重复的细胞分裂。