Comparative Gastrointestinal and Renal Transport Physiology of Crustaceans

甲壳类动物胃肠道和肾脏运输生理学的比较

• 批准号: 8809930
• 负责人: Gregory Ahearn
• 金额: $ 6.35万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-15 至 1989-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8809930&HistoricalAwards=false
• 关键词: Comparative; Gastrointestinal; Renal; Transport; Physiology

Dr. Ahearn plans to continue studies on the mechanisms of solute transport by crustacean gastrointestinal and renal epithelial cells. The two major goals are: 1) Continue state-of-the-art membrane vesicle studies designed to elucidate the mechanisms of ion, amino acid, sugar, lipid, peptide, vitamin, and organic acid transport across isolated and purified brush border and basolateral membranes of crustacean hepatopancreatic and antennal gland epithelia; and 2) Establish preliminary data showing the feasibility of growing functional epithelial cell monolayers from crustacean hepatopancreas and/or antennal gland that can be used in transepithelial transport studies of inorganic and organic solutes. The combination of these two approaches to epithelial biology should lead to significant information about the mechanisms of nutrient and ion absorption and organic acid secretion in two major organ systems of this economically- important group of invertebrates.

Ahearn博士计划继续研究溶质 甲壳类胃肠和肾上皮转运 细胞 两个主要目标是：1）继续保持最先进水平 膜囊泡研究旨在阐明 离子、氨基酸、糖、脂质、肽、维生素和有机酸 穿越隔离和净化的灌木丛边界， 甲壳动物肝胰腺和触角的底侧膜 腺上皮;和2）建立初步数据显示， 培养功能性上皮细胞单层的可行性 可使用的甲壳动物肝胰腺和/或触角腺 在无机和有机的跨上皮转运研究中， 溶质。 这两种方法的结合对上皮细胞 生物学应该会带来关于 养分和离子吸收机制及有机酸 在两个主要器官系统中的分泌， 无脊椎动物的重要类群。