Comparative Gastrointestinal and Renal Transport Physiology of Crustaceans

埃亨博士计划继续研究甲壳类动物胃肠道和肾上皮细胞的溶质运输机制。两个主要目标是：1)继续最先进的膜囊研究，旨在阐明离子、氨基酸、糖、脂、肽、维生素和有机酸跨分离和纯化的甲壳动物肝胰腺和触角腺上皮的刷缘和基侧膜转运的机制；2)建立甲壳动物肝胰腺和/或触角腺培养功能性上皮细胞单层的初步数据，该单层可用于无机和有机溶质的跨上皮运输研究。这两种上皮生物学方法的结合应该会带来关于这一经济上重要的无脊椎动物群体的两个主要器官系统中营养和离子吸收以及有机酸分泌机制的重要信息。

Dr. Ahearn plans to continue studies on the mechanisms of solute transport by crustacean gastrointestinal and renal epithelial cells. The two major goals are: 1) Continue state-of-the-art membrane vesicle studies designed to elucidate the mechanisms of ion, amino acid, sugar, lipid, peptide, vitamin, and organic acid transport across isolated and purified brush border and basolateral membranes of crustacean hepatopancreatic and antennal gland epithelia; and 2) Establish preliminary data showing the feasibility of growing functional epithelial cell monolayers from crustacean hepatopancreas and/or antennal gland that can be used in transepithelial transport studies of inorganic and organic solutes. The combination of these two approaches to epithelial biology should lead to significant information about the mechanisms of nutrient and ion absorption and organic acid secretion in two major organ systems of this economically- important group of invertebrates.