Mechanisms of Intestinal Nutrient Transport in Fish

鱼类肠道营养物质运输机制

• 批准号: 8715278
• 负责人: Gregory Ahearn
• 金额: $ 26.73万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8715278&HistoricalAwards=false
• 关键词: Mechanisms; Intestinal; Nutrient; Transport; Fish

The proposed 3-year investigation is a request for a continuation of a program charactering-important fish species. The investigators will use state-of-the-art membrane vesicle techniques to elucidate the mechanisms of amino acid, short-chain fatty acid, peptide, and vitamin uptake into intestinal and pyloric cecal epithelial cells of the trout and tilapia in Hawaii and the upper and lower intestinal segments of the eel in Italy. In addition, cell culture methods will be used to grow confluent epithelial monolayers from intestine and pyloric ceca of the respective fishes which can be mounted in Ussing chambers to investigate the processes of transepithelial nutrient transport. This study should lead to important information about the mechanisms of nutrient absorption in herbivorous (tilapia) and carnivorous (trout, eel) fish species, and should elucidate the relative importance of intestinal and pyloric cecal epithelia in these absorptive processes. The study has application value ecomonics of food utilization in the fish farming operations.

拟议的为期3年的调查是对重要鱼类物种特征的计划的继续。研究人员将使用最先进的膜泡囊技术来阐明氨基酸、短链脂肪酸、多肽和维生素在夏威夷的鲑鱼和罗非鱼以及意大利鳗鱼的上下肠段的肠道和幽门盲肠上皮细胞中的摄取机制。此外，将使用细胞培养方法从各自鱼的肠道和幽门盲囊培养融合上皮单层，这些单层可以安装在Ussing小室中，以研究跨上皮营养物质的运输过程。这项研究将提供有关草食性(罗非鱼)和肉食性(鲑鱼、鳗鱼)鱼种营养吸收机制的重要信息，并应阐明肠道和幽门盲肠上皮在这些吸收过程中的相对重要性。本研究对养鱼作业中食物利用的生态化具有应用价值。